Effect of acid etching time on demineralization of primary and permanent coronal dentin

Author(s)

Salles Scheffel, Debora Lopes ^[1] ; Andalo Tenuta, Livia Maria ^[2] ; Cury, Jaime Aparecido ^[2] ; Hebling, Josimeri ^[1]

Affiliation

^[1] Univ Estadual Paulista, UNESP, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Sao Paulo - Brazil

• Document type: Journal article
• Source: AMERICAN JOURNAL OF DENTISTRY; v. 25, n. 4, p. 235-238, AUG 2012.
• Web of Science Citations: 5

Abstract

Purpose: To determine the effect of acid etching time on dentin calcium solubilization and to compare the solubilization rate of primary and permanent dentin when etched for the same period of time. Methods: Flat coronal dentin surfaces were produced on primary (n=8) and permanent (n=8) sound teeth. Three 2 mm-diameter areas were delimited on each surface and assigned to 5, 10 or 15 seconds of acid etching. Five microliters of 35% H3PO4 were applied on each area during the preset times, and 4 mu L, were retrieved for analysis. The amount of calcium was determined colorimetrically using Arsenazo III reagent and expressed as mu g Ca/mm(2) of dentin. Data were subjected to two-way ANOVA, Tukey's test and linear regression (alpha=5%). Results: For both primary and permanent dentin, a statistically significant correlation was observed between acid etching time and calcium concentration (primary dentin R-2= 0.79; permanent dentin R-2= 0.73). Mean (sd) values of dissolved primary dentin after 5, 10 and 15 seconds were 1.00 (0.25), 1.74 (0.11) and 2.30 (0.42), whereas for permanent dentin the values were 0.47 (0.13), 0.89 (0.36) and 1.38 (0.20) mu g Ca/mm(2), respectively. Greater calcium solubilization (P< 0.05) was detected for primary dentin than for permanent dentin after all acid etching times. (Am J Dent 2012;25:235-238). (AU)

• FAPESP's process: 09/07924-8 - Acid etching time of sound and caries-affected dentin of decidous and permanent teeth: effect on the substrate solubility and production of the resin-dentin bond
• Grantee: Josimeri Hebling Costa
• Support type: Regular Research Grants
• FAPESP's process: 08/02278-8 - Acid etching time of sound and caries-affected dentin of deciduous and permanent teeth: effect on substrate dissolution and resin-dentin bond strength
• Grantee: Débora Lopes Salles Scheffel
• Support type: Scholarships in Brazil - Master