NEW MICROSATELLITE MARKERS FOR GARLIC, ALLIUM SATIVUM (ALLIACEAE)

Autor(es)

Cunha, Camila P. ^[1] ; Hoogerheide, Eulalia S. S. ^[2] ; Zucchi, Maria I. ^[3] ; Monteiro, Mariza ^[3] ; Pinheiro, Jose B. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, BR-13400970 Sao Paulo - Brazil

^[2] Embrapa Agrossilvipastoril, BR-78550003 Sinop, Mato Grosso - Brazil

^[3] Agencia Paulista Tecnol Agronegoc, BR-13400970 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AMERICAN JOURNAL OF BOTANY; v. 99, n. 1, p. E17-E19, JAN 2012.
• Citações Web of Science: 16

Resumo

Premise of the study: A new set of microsatellite or simple sequence repeat (SSR) markers for garlic, an important medicinal spice, was developed to aid studies of genetic diversity and to define efficient strategies for germplasm conservation. Methods and Results: Using a (CT)(8)- and (GT)(8)-enriched library, a total of 16 SSR loci were developed and optimized in garlic. Ten loci were found to be polymorphic after screening 75 accessions. The parameters used to characterize the loci were observed and expected heterozygosity, number of alleles, Shannon Index, and polymorphism information content (PIC). A total of 44 alleles were identified, with an average of 4.4 alleles per loci. The vast majority of loci were moderate to highly informative according to PIC and the Shannon Index. Conclusion: The new SSR markers have the potential to be informative tools for genetic diversity, allele mining, mapping and associative studies, and in the management and conservation of garlic collections. (AU)

• Processo FAPESP: 08/02936-5 - Desenvolvimento de biblioteca enriquecida com locos SSRs e caracterização agromorfológica e molecular do germoplasma de Allium sativum L.
• Beneficiário: José Baldin Pinheiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/03577-1 - Caracterização de germoplasma de alho (Allium sativum L.) através de marcadores moleculares microssatélites
• Beneficiário: Camila Pinto da Cunha
• Modalidade de apoio: Bolsas no Brasil - Mestrado