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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

NEW MICROSATELLITE MARKERS FOR GARLIC, ALLIUM SATIVUM (ALLIACEAE)

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Author(s):
Cunha, Camila P. [1] ; Hoogerheide, Eulalia S. S. [2] ; Zucchi, Maria I. [3] ; Monteiro, Mariza [3] ; Pinheiro, Jose B. [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, BR-13400970 Sao Paulo - Brazil
[2] Embrapa Agrossilvipastoril, BR-78550003 Sinop, Mato Grosso - Brazil
[3] Agencia Paulista Tecnol Agronegoc, BR-13400970 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: AMERICAN JOURNAL OF BOTANY; v. 99, n. 1, p. E17-E19, JAN 2012.
Web of Science Citations: 16
Abstract

Premise of the study: A new set of microsatellite or simple sequence repeat (SSR) markers for garlic, an important medicinal spice, was developed to aid studies of genetic diversity and to define efficient strategies for germplasm conservation. Methods and Results: Using a (CT)(8)- and (GT)(8)-enriched library, a total of 16 SSR loci were developed and optimized in garlic. Ten loci were found to be polymorphic after screening 75 accessions. The parameters used to characterize the loci were observed and expected heterozygosity, number of alleles, Shannon Index, and polymorphism information content (PIC). A total of 44 alleles were identified, with an average of 4.4 alleles per loci. The vast majority of loci were moderate to highly informative according to PIC and the Shannon Index. Conclusion: The new SSR markers have the potential to be informative tools for genetic diversity, allele mining, mapping and associative studies, and in the management and conservation of garlic collections. (AU)

FAPESP's process: 08/02936-5 - Development of library enriched with locus SSRs and agromorphologic and molecular characterization of the Allium sativum L. germoplasm
Grantee:José Baldin Pinheiro
Support Opportunities: Regular Research Grants
FAPESP's process: 09/03577-1 - Microsatellite markers for genetic analysis of GARLIC(Allium sativum L.)GERMPLASM
Grantee:Camila Pinto da Cunha
Support Opportunities: Scholarships in Brazil - Master