Cytokeratin Profile in Exfoliative Cytology of Smokers

Autor(es)

Lima, Celina Faig ^[1] ; Pinotti Leite, Sabrina Ferreira ; Carvalho, Yasmin Rodarte ; Guimaraes Cabral, Luiz Antonio ; Balducci, Ivan ^[2] ; Almeida, Janete Dias

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Sao Jose dos Campos Dent Sch, Dept Biosci & Oral Diag, Postgrad Program Oral Biopathol, BR-12245000 Sao Paulo - Brazil

^[2] Sao Paulo State Univ, Sao Jose dos Campos Dent Sch, Dept Social Sci & Pediat Dent, BR-12245000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANALYTICAL AND QUANTITATIVE CYTOLOGY AND HISTOLOGY; v. 33, n. 1, p. 19-24, FEB 2011.
• Citações Web of Science: 3

Resumo

OBJECTIVE: To compare the expression of cytokeratins (CKs) 6, 16, 19 and pan-cytokeratin (PAN) in oral mucosa cells between smokers and nonsmokers to determine the proliferative activity and expression indicative of a potential for malignant transformation. STUDY DESIGN: Smears were obtained from the kit lateral border of the tongue with a cytobrush from 25 smokers and 20 nonsmokers seen at the clinics of Silo Jose dos Campos Dental School, Sao Paulo State University, Sao Jose dos Campos, Silo Paulo, Brazil, and processed for immunohistochemistry. Conventional microscopy was used for qualitative analysis. Proportions were compared statistically by the z-test and Fisher's exact test. RESULTS: The expression of CK6 (p = 0.002), CK16 (p = 0.003), CK19 ( p = 0.0001) and PAN (p = 0.008) was higher in oral mucosa smears from smokers compared to nonsmokers. CONCLUSION: The expression of CK6 and CK16 demonstrated increased epithelial proliferation in the oral mucosa of smokers, and expression of CK19 indicated alterations in epithelial maturation. The expression of PAN indicates the need for the investigation of other types of CK in further studies. (Anal Quant Cytol Histol 2011;33:19-24) (AU)

• Processo FAPESP: 04/03839-2 - Manifestações bucais do Diabetes Mellitus tipo I e II em usuários de próteses totais
• Beneficiário: Danielle Cristina de Lima
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 04/03838-6 - Manifestações bucais do Diabetes Mellitus tipo I e II em usuários de próteses totais
• Beneficiário: Gislaine Cristina Nakata
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica