Phosphoproteome Reveals an Atlas of Protein Signaling Networks During Osteoblast Adhesion

Autor(es):Mostrar menos -

Milani, Renato ; Ferreira, Carmen V. ; Granjeiro, Jose M. ^[1] ; Paredes-Gamero, Edgar J. ^[2] ; Silva, Rodrigo A. ; Justo, Giselle Z. ^[2] ; Nader, Helena B. ^[2] ; Galembeck, Eduardo ; Peppelenbosch, Maikel P. ^[3] ; Aoyama, Hiroshi ; Zambuzzi, Willian F. ^[4]

Afiliação do(s) autor(es)

^[1] Univ Fed Fluminense, Inst Biol, Dept Cellular & Mol Biol, BR-24050120 Rio De Janeiro - Brazil

^[2] Univ Fed Sao Paulo, Dept Biophys, BR-04044020 Sao Paulo - Brazil

^[3] Univ Groningen, Univ Med Ctr Groningen, Dept Cell Biol, Kinome Profiling Unit, NL-9713 AV Groningen - Netherlands

^[4] Univ Estadual Campinas, Div Cell Signaling & Bioassays, Inst Biol, Dept Biochem, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Cellular Biochemistry; v. 109, n. 5, p. 957-966, APR 1 2010.
• Citações Web of Science: 27

Resumo

Cell adhesion on surfaces is a fundamental process in the emerging biomaterials field and developmental events as well. However, the mechanisms regulating this biological process in osteoblasts are not fully understood. Reversible phosphorylation catalyzed by kinases is probably the most important regulatory mechanism in eukaryotes. Therefore, the goal of this study is to assess osteoblast adhesion through a molecular prism under a peptide array technology, revealing essential signaling proteins governing adhesion-related events. First, we showed that there are main morphological changes on osteoblast shape during adhesion up to 3 h. Second, besides classical proteins activated upon integrin activation, our results showed a novel network involving signaling proteins such as Rap1A, PKA, PKC, and GSK3 beta during osteoblast adhesion on polystyrene. Third, these proteins were grouped in different signaling cascades including focal adhesion establishment, cytoskeleton rearrangement, and cell-cycle arrest. We have thus provided evidence that a global phosphorylation screening is able to yield a systems-oriented look at osteoblast adhesion, providing new insights for understanding of bone formation and improvement of cell-substratum interactions. Altogether, these statements are necessary means for further intervention and development of new approaches for the progress of tissue engineering. Cell. Biochem. 109: 957-966, 2010. (C) 2010 Wiley-Liss, Inc. (AU)

• Processo FAPESP: 08/53003-9 - Investigação da função da proteína tirosina fosfatase de baixa massa molecular (LMW-PTP) na adesão de osteoblastos e formação óssea
• Beneficiário: Willian Fernando Zambuzzi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado