MMP-2 is not Altered By Stretching in Skeletal Muscle

Autor(es)

Peviani, S. M. ^[1] ; Gomes, A. R. S. ^[2] ; de Araujo, H. S. Selistre ^[3] ; Salvini, T. F. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Phys Therapy, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Fed Parana, Dept Phys Therapy, Matinhos - Brazil

^[3] Univ Fed Sao Carlos, Dept Physiol, BR-13560 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF SPORTS MEDICINE; v. 30, n. 7, p. 550-554, JUL 2009.
• Citações Web of Science: 4

Resumo

Considering that short bouts of stretching, as recommended in rehabilitation and sports activities, induce skeletal Muscle and connective tissue adaptation, the hypothesis of this study was that MMP-2 activity is regulated by muscle stretch. The level of MMP-2 activity was, thereby, assessed after stretching in rat soleus muscle. Animals received a single session of stretching (10 stretches lasting 1 min each with 30s of rest in between) and were evaluated immediately and after 8, 24, 48, 72 and 168 h. To evaluate the effect of repetitive sessions of stretching, three groups of animals were evaluated - one group after 2 sessions, another after 3, and a third after 7. MMP-2 activity was evaluated by zymography and MMP-2 mRNA was assessed by real-time polymerase chain reaction. None of the groups presented MMP-9 activity. MMP-2 activity and mRNA expression did not change after either single or repetitive sessions of stretching. In conclusion, the results of this study indicate that MMP-2 is not involved in the muscle stretch-induced remodeling. (AU)

• Processo FAPESP: 05/55773-8 - O efeito do alongamento intermitente na expressão gênica e na atividade de metaloproteases na matriz extraceular do músculo esquelético
• Beneficiário: Sabrina Peviani
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 03/10889-3 - O efeito do alongamento muscular na expressao genica dos fatores regulatorios miogenicos (mrfs).
• Beneficiário: Tania de Fatima Salvini
• Modalidade de apoio: Auxílio à Pesquisa - Regular