Genetic diversity, virulence genes and antimicrobial resistance of Salmonella Enteritidis isolated from food and humans over a 24-year period in Brazil

Autor(es)

Campioni, Fabio ^[1] ; Moratto Bergamini, Alzira Maria ^[2] ; Falcao, Juliana P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Adolfo Lutz Inst Ribeirao Preto, BR-14085410 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FOOD MICROBIOLOGY; v. 32, n. 2, p. 254-264, DEC 2012.
• Citações Web of Science: 49

Resumo

Salmonellosis is a major health problem worldwide. Serovar Enteritidis has been a primary cause of Salmonella outbreaks in many countries. In Brazil, few molecular typing studies have been performed. The aims of this study were to molecularly type Salmonella Enteritidis strains isolated in Brazil in order to determine the genetic relationship between strains of food and human origin, as well as, to assess their pathogenic potential and antimicrobial resistance. A total of 128 S. Enteritidis strains isolated from human feces (67) and food (61) between 1986 and 2010 were studied. The genotypic diversity was assessed by ERIC-PCR and PFGE using Xbal, the antimicrobial resistance by the disc-diffusion assay and the presence of the SPI-1, SPI-2 and pSTV virulence genes assessed by PCR. The ERIC-PCR results revealed that 112 strains exhibited a similarity of >85.4% and the PFGE that 96 strains exhibited a similarity of >80.0%. Almost all strains (97.6%) harbored all 13 virulence genes investigated. Thirty-six strains (28.12%) were resistant to nalidixic acid. In conclusion, the nalidixic acid resistance observed after 1996 is indicative of an increase in the use of this drug. It may be suggested that these 128 strains might have descended from a common ancestor that differed little over 24 years and has been both contaminating food and humans and causing disease for more than two decades in Brazil. (c) 2012 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 08/57478-1 - Tipagem molecular e analise da diversidade genetica de linhagens de salmonella enteritidis isoladas de humanos e de alimentos no estado de sao paulo, brasil.
• Beneficiário: Juliana Pfrimer Falcão
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/09998-9 - Tipagem molecular e análise da diversidade genética de linhagens de Salmonella Enteritidis isoladas de humanos e de alimentos no Estado de São Paulo, Brasil.
• Beneficiário: Fábio Campioni
• Modalidade de apoio: Bolsas no Brasil - Doutorado