Involvement of PGE(2) and RANTES in Staphylococcus aureus-induced fever in rats

Autor(es)

Martins, Juliano M. ^[1] ; Longhi-Balbinot, Daniela T. ^[1] ; Soares, Denis M. ^[1] ; Figueiredo, Maria Jose ^[1] ; Malvar, David do C. ^[1] ; de Melo, Miriam C. C. ^[1] ; Rae, Giles A. ^[2] ; Souza, Gloria E. P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Pharmacol Lab, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Santa Catarina, Dept Pharmacol, Ctr Biol Sci, Florianopolis, SC - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Physiology; v. 113, n. 9, p. 1456-1465, NOV 2012.
• Citações Web of Science: 3

Resumo

Martins JM, Longhi-Balbinot DT, Soares DM, Figueiredo MJ, Malvar D do C, de Melo MC, Rae GA, Souza GE. Involvement of PGE(2) and RANTES in Staphylococcus aureus-induced fever in rats. J Appl Physiol 113: 1456-1465, 2012. First published August 30, 2012; doi:10.1152/japplphysiol.00936.2011.-This study investigated the involvement of prostaglandins and regulated on activation, normal T cell expressed and secreted (RANTES), in fever induced by live Staphylococcus aureus (no. 25923, American Type Culture Collection) injection in rats. S. aureus was injected intraperitoneally at 10(9), 10(10), and 2 x 10(10) colony-forming units (CFU)/cavity, and body temperature (T-b) was measured by radiotelemetry. The lowest dose of S. aureus induced a modest transient increase in T-b, whereas the two higher doses promoted similar long-lasting and sustained T-b increases. Thus, the 10(10) CFU/cavity dose was chosen for the remaining experiments. The T-b increase induced by S. aureus was accompanied by significant decreases in tail skin temperature and increases in PGE(2) levels in the cerebrospinal fluid (CSF) and hypothalamus but not in the venous plasma. Celecoxib (selective cyclooxygenase-2 inhibitor, 2.5 mg/kg po) inhibited the fever and the increases in PGE(2) concentration in the CSF and hypothalamus induced by S. aureus. Dipyrone (120 mg/kg ip) reduced the fever from 2.5 to 4 h and the PGE(2) increase in the CSF but not in the hypothalamus. S. aureus increased RANTES in the peritoneal exudate but not in the CSF or hypothalamus. Met-RANTES (100 mu g/kg iv), a chemokine (C-C motif) receptor (CCR)1/CCR5 antagonist, reduced the first 6 h of fever induced by S. aureus. This study suggests that peripheral (local) RANTES and central PGE(2) production are key events in the febrile response to live S. aureus injection. As dipyrone does not reduce PGE(2) synthesis in the hypothalamus, it is plausible that S. aureus induces fever, in part, via a dipyrone-sensitive PGE(2)-independent pathway. (AU)

• Processo FAPESP: 07/04791-1 - Isolamento e caracterização bioquímica da fração responsável pelo efeito pirogênico e nociceptivo presente no veneno do escorpião Tityus serrulatus
• Beneficiário: Glória Emília Petto de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/04860-0 - Mediação da resposta febril induzida pela injeção de Staphylococcus aureus em ratos
• Beneficiário: Juliano Manvailer Martins
• Modalidade de apoio: Bolsas no Brasil - Doutorado