Susceptibility of neuron-like cells derived from bovine Wharton's jelly to bovine herpesvirus type 5 infections

Autor(es)

Cardoso, Tereza C. ^[1] ; Novais, Juliana B. ^[1] ; Antello, Talita F. ^[1] ; Silva-Frade, Camila ^[1] ; Ferrarezi, Marina C. ^[1] ; Ferrari, Heitor F. ^[1] ; Gameiro, Roberto ^[2] ; Flores, Eduardo F. ^[3]

Afiliação do(s) autor(es)

^[1] UNESP Univ Sao Paulo State, Lab Anim Virol & Cell Culture, BR-16050680 Sao Paulo, Aracatuba - Brazil

^[2] UNESP Univ Sao Paulo State, Embryol Lab, Fac Vet Med, BR-16050680 Sao Paulo, Aracatuba - Brazil

^[3] Univ Fed Santa Maria, UNESP Univ Sao Paulo State, Virol Sect, BR-97115900 Santa Maria, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC Veterinary Research; v. 8, DEC 10 2012.
• Citações Web of Science: 5

Resumo

Background: Bovine herpesvirus type 5 (BoHV-5), frequently lethal in cattle, is associated with significant agricultural economic losses due to neurological disease. Cattle and rabbits are frequently used as models to study the biology and pathogenesis of BoHV-5 infection. In particular, neural invasion and proliferation are two of the factors important in BoHV-5 infection. The present study investigated the potential of bovine Wharton's jelly mesenchymal stromal cells (bWJ-MSCs) to differentiate into a neuronal phenotype and support robust BoHV-5 replication. Results: Upon inducing differentiation within a defined neuronal specific medium, most bWJ-MSCs acquired the distinctive neuronal morphological features and stained positively for the neuronal/glial markers MAP2 (neuronal microtubule associated protein 2), N200 (neurofilament 200), NT3 (neutrophin 3), tau and GFAP (glial fibrillary acidic protein). Expression of nestin, N200, beta-tubulin III (TuJI) and GFAP was further demonstrated by reverse transcriptase polymerase chain reaction (RT-PCR). Following BoHV-5 inoculation, there were low rates of cell detachment, good cell viability at 96 h post-infection (p.i.), and small vesicles developed along neuronal branches. Levels of BoHV-5 antigens and DNA were associated with the peak in viral titres at 72 h p.i. BoHV-5 glycoprotein C mRNA expression was significantly correlated with production of progeny virus at 72 h p.i. (p < 0.05). Conclusion: The results demonstrated the ability of bWJ-MSCs to differentiate into a neuronal phenotype in vitro and support productive BoHV-5 replication. These findings constitute a remarkable contribution to the in vitro study of neurotropic viruses. This work may pave the way for bWJ-MSCs to be used as an alternative to animal models in the study of BoHV-5 biology. (AU)

• Processo FAPESP: 09/17635-3 - Estudo epidemiológico, anátomo-patológico e molecular da meningoencefalite não supurativa ocasionada pelo herpesvírus tipo 5
• Beneficiário: Tereza Cristina Cardoso da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/50782-7 - Susceptibilidade de células embrionárias de perus (mesenquimais respiratórias e digestivas) à infecção in vitro pelo coronavírus tipo III
• Beneficiário: Tereza Cristina Cardoso da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular