Molecular phylogenetics of Oestroidea (Diptera: Calyptratae) with emphasis on Calliphoridae: Insights into the inter-familial relationships and additional evidence for paraphyly among blowflies

Autor(es)

Marinho, M. A. T. ^[1] ; Junqueira, A. C. M. ; Paulo, D. F. ; Esposito, M. C. ^[2] ; Villet, M. H. ^[3] ; Azeredo-Espin, A. M. L. ^[4]

Afiliação do(s) autor(es)

^[1] Campinas State Univ UNICAMP, Ctr Mol Biol & Genet Engn CBMEG, Lab Anim Genet & Evolut, BR-13083875 Campinas, SP - Brazil

^[2] Fed Univ Para, Inst Biol Sci, BR-66059 Belem, Para - Brazil

^[3] Rhodes Univ, Dept Zool & Entomol, So African Forens Entomol Res Lab, ZA-6140 Grahamstown - South Africa

^[4] Campinas State Univ UNICAMP, Inst Biol, Dept Genet & Evolut, BR-13083875 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecular Phylogenetics and Evolution; v. 65, n. 3, p. 840-854, DEC 2012.
• Citações Web of Science: 41

Resumo

The superfamily Oestroidea, comprising similar to 15,000 species, is a large and ecologically diverse clade within the order Diptera. Among its six commonly recognized families, Calliphoridae seems to be crucial for understanding evolutionary relationships in the group, as it is recognized as a controversial paraphyletic grouping. To further investigate this matter, the ITS2, 28S, COI and 16S regions were used to infer phylogenetic relationships in Oestroidea with maximum-parsimony (MP), maximum-likelihood (ML) and Bayesian inference (BI) methods. For the BI analyses, a deep evaluation of different data partitioning strategies was conducted, including consideration of structural conformation (ITS2 and 16S) and codon position (COI) information. Results suggest the existence of two main clades in Oestroidea: (Tachinidae + Mesembrinellinae) and (Rhiniinae, (Sarcophagidae + Calliphoridae sensu stricto)). Oestridae was recovered as sister group of the remaining Oestroidea in the MP trees while it was placed closer to the (Rhiniinae + Sarcophagidae + Calliphoridae sensu stricto) group in the ML and BI trees. A paraphyletic Calliphoridae was recovered, confirming the exclusion of Rhiniinae, a clade recently promoted to family status and therefore already excluded. Mesembrinellinae could also be considered a distinct group apart from Calliphoridae, although further studies are required. Consideration of structural and codon position information led to a significant increase in the log-likelihoods of the analyses, which were accompanied by small changes in the inferred topologies, branch lengths and posterior probability support values. However, as model complexity increases, so does uncertainty across the estimated parameters, including tree topologies, and phylogenies inferred under very parameter-rich models may be less reliable even when possessing higher log-likelihoods. (c) 2012 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 08/56769-2 - Genômica mitocondrial e filogenia molecular do grupo Schizophora (Diptera: Brachycera) utilizando sequenciamento de nova geração
• Beneficiário: Ana Carolina Martins Junqueira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 06/61217-3 - Organização e estrutura do ITS2 em Calyptratae (Diptera: Brachycera) e sua implicação em estudos genético-evolutivos em moscas causadoras de miíases da família Calliphoridae
• Beneficiário: Marco Antonio Tonus Marinho
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto