Phylogenetic relationship of dengue virus type 3 isolated in Brazil and Paraguay and global evolutionary divergence dynamics

Autor(es):Mostrar menos -

Alfonso, Helda Liz ^[1] ; Amarilla, Alberto Anastacio ^[1] ; Goncalves, Paula Fernanda ^[1] ; Barros, Matheus Takatuba ^[1] ; de Almeida, Flavia Tremeschin ^[1] ; Silva, Telma R. ^[1] ; da Silva, Eliana V. ^[2] ; Nunes, Marcio T. ^[2] ; Vasconcelos, Pedro F. C. ^[2] ; Vieira, Deusilene S. ^[3] ; Batista, Weber Cheli ^[3] ; Bobadilla, Maria Liz ^[4] ; Vazquez, Cynthia ^[4] ; Moran, Mirian ^[4] ; Moraes Figueiredo, Luiz Tadeu ^[5] ; Aquino, Victor Hugo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Inst Evandro Chagas, Dept Arbovirol & Febres Hemorrag, BR-67030000 Ananindeua, PA - Brazil

^[3] Fdn Oswaldo Cruz Noroeste, Inst Pesquisas Patologias Trop Rondonia, BR-76800000 Porto Velho, RO - Brazil

^[4] Minist Salud & Bienestar Social, Dept Virol, Lab Cent Salud Publ, Asuncion - Paraguay

^[5] Univ Sao Paulo, Fac Med Ribeirao Preto, Ctr Pesquisa Virol, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VIROLOGY JOURNAL; v. 9, JUN 20 2012.
• Citações Web of Science: 5

Resumo

Background: Dengue is the most important mosquito-borne viral disease worldwide. Dengue virus comprises four antigenically related viruses named dengue virus type 1 to 4 (DENV1-4). DENV-3 was re-introduced into the Americas in 1994 causing outbreaks in Nicaragua and Panama. DENV-3 was introduced in Brazil in 2000 and then spread to most of the Brazilian States, reaching the neighboring country, Paraguay in 2002. In this study, we have analyzed the phylogenetic relationship of DENV-3 isolated in Brazil and Paraguay with viruses isolated worldwide. We have also analyzed the evolutionary divergence dynamics of DENV-3 viruses. Results: The entire open reading frame (ORF) of thirteen DENV-3 isolated in Brazil (n = 9) and Paraguay (n = 4) were sequenced for phylogenetic analysis. DENV-3 grouped into three main genotypes (I, II and III). Several internal clades were found within each genotype that we called lineage and sub-lineage. Viruses included in this study belong to genotype III and grouped together with viruses isolated in the Americas within the lineage III. The Brazilian viruses were further segregated into two different sub-lineage, A and B, and the Paraguayan into the sub-lineage B. All three genotypes showed internal grouping. The nucleotide divergence was in average 6.7% for genotypes, 2.7% for lineages and 1.5% for sub-lineages. Phylogenetic trees constructed with any of the protein gene sequences showed the same segregation of the DENV-3 in three genotypes. Conclusion: Our results showed that two groups of DENV-3 genotypes III circulated in Brazil during 2002-2009, suggesting different events of introduction of the virus through different regions of the country. In Paraguay, only one group DENV-3 genotype III is circulating that is very closely related to the Brazilian viruses of sub-lineage B. Different degree of grouping can be observed for DENV-3 and each group showed a characteristic evolutionary divergence. Finally, we have observed that any protein gene sequence can be used to identify the virus genotype. (AU)

• Processo FAPESP: 10/50432-6 - Estudo do dengue nas Regiões Norte e Sudeste do Brasil: criação de uma rede interdisciplinar de pesquisa básica aplicada
• Beneficiário: Luiz Tadeu Moraes Figueiredo
• Modalidade de apoio: Auxílio à Pesquisa - Temático