Novel natural genetic variation controlling the competence to form adventitious roots and shoots from the tomato wild relative Solanum pennellii

Autor(es)

Arikita, Fernanda Namie ^[1] ; Azevedo, Mariana Silva ^{[1, 2]} ; Scotton, Danielle Camargo ^{[1, 2]} ; Pinto, Maisa de Siqueira ^[1] ; Figueira, Antonio ^[2] ; Pereira Peres, Lazaro Eustaquio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz ESALQ, Dept Biol Sci LCB, Lab Hormonal Control Plant Dev, BR-13418900 Piracicaba, SP - Brazil

^[2] Univ Sao Paulo, Ctr Energia Nucl Agr CENA, BR-13400970 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Plant Science; v. 199, p. 121-130, FEB 2013.
• Citações Web of Science: 9

Resumo

Tomato (Solanum lycopersicum L.) is an attractive model to study the genetic basis of adventitious organ formation capacity, since there is considerable natural genetic variation among wild relatives. Using a set of 46 introgression lines (ILs), each containing a small chromosomal segment of Solanum pennellii LA716 introgressed and mapped into the tomato cultivar M82, we characterized a high shoot-regeneration capacity for ILs 3-2, 6-1, 7-1, 7-2, 8-2, 8-3, 9-1, 9-2, 10-2 and 10-3, when cotyledon explants were cultivated on medium containing 5.0 mu M BAP. F1 seedlings from the crosses `Micro-Tom x ILs' and `ILs x ILs' demonstrated that the shoot regeneration capacity of most ILs was dominant and that the regeneration ability of IL8-3 enhanced that of the other ILs in an additive manner. The ILs 3-2, 7-1, 8-3, and 10-2 also exhibited enhanced root formation on MS medium containing 0.4 mu M NAA, indicating that these chromosomal segments may contain genes controlling the competence to assume distinct cell fates, rather than the induction of a specific organ. We also performed the introgression of the genes controlling competence into the model system `Micro-Tom'. The further isolation of such genes will improve our understanding of the molecular basis of organogenic capacity. (c) 2012 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 07/07175-0 - Análise genética da resposta de defesa de Theobroma cacao a Moniliophthora perniciosa, agente causal da vassoura-de-bruxa
• Beneficiário: Antonio Vargas de Oliveira Figueira
• Modalidade de apoio: Auxílio à Pesquisa - Temático