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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

VEGF-C expression in oral cancer by neurotransmitter-induced activation of beta-adrenergic receptors

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Autor(es):
Rodrigues Vilardi, Bruna Maria [1] ; Bravo-Calderon, Diego Mauricio [1] ; Bernabe, Daniel Galera [2, 3] ; Penha Oliveira, Sandra Helena [4] ; Oliveira, Denise Tostes [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Bauru Sch Dent, Dept Stomatol, Area Pathol, BR-17012901 Sao Paulo - Brazil
[2] Univ Estadual Paulista UNESP, Sch Dent Aracatuba, Oral Oncol Ctr, Sao Paulo - Brazil
[3] Univ Estadual Paulista UNESP, Sch Dent Aracatuba, Dept Pathol & Clin Propedeut, Sao Paulo - Brazil
[4] Univ Estadual Paulista UNESP, Sch Dent Aracatuba, Dept Basic Sci, Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: TUMOR BIOLOGY; v. 34, n. 1, p. 139-143, FEB 2013.
Citações Web of Science: 5
Resumo

The aim of this study was to investigate the expression of vascular endothelial growth factor type C (VEGF-C) in oral squamous cell carcinoma (OSCC) cell lines through norepinephrine-induced activation of beta-adrenergic receptors. Human OSCC cell lines (SCC-9 and SCC-25) expressing beta-adrenergic receptors were stimulated with different concentrations of norepinephrine (0.1, 1, and 10 mu M) and 1 mu M of propranolol, and analyzed after 1, 6, and 24 h. VEGF-C gene expression and VEGF-C production in the cell supernatant were evaluated by real-time PCR and by ELISA, respectively. The results showed that beta-adrenergic receptor stimulation by different concentrations of norepinephrine or blocking by propranolol did not markedly alter VEGF-C expression by SCC-9 and SCC-25 cells. VEGF-C protein levels produced by oral malignant cell lines after stimulation with different norepinephrine concentrations or blocking with propranolol was statistically similar (p > 0.05) to those of the control group (nonstimulated OSCC cell lines). Our findings suggest that stimulation of beta-adrenergic receptors by means of norepinephrine does not seem to modulate the VEGF-C expression in OSCC cell lines. These findings reinforce the need for further studies in order to understand the responsiveness of oral cancer to beta-adrenergic receptor stimulation or blockage, especially with regard to VEGF-C production. (AU)

Processo FAPESP: 10/06333-3 - Modulação da expressão de VEGF-C por mediador relacionado ao estresse em cultura de carcinoma espinocelular de boca
Beneficiário:Denise Tostes Oliveira
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 10/51361-5 - Análise dos efeitos do estresse crônico e da regulação neuroendócrina sobre o câncer de boca
Beneficiário:Daniel Galera Bernabé
Linha de fomento: Bolsas no Brasil - Pós-Doutorado