VEGF-C expression in oral cancer by neurotransmitter-induced activation of beta-adrenergic receptors

Autor(es)

Rodrigues Vilardi, Bruna Maria ^[1] ; Bravo-Calderon, Diego Mauricio ^[1] ; Bernabe, Daniel Galera ^{[2, 3]} ; Penha Oliveira, Sandra Helena ^[4] ; Oliveira, Denise Tostes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Stomatol, Area Pathol, BR-17012901 Sao Paulo - Brazil

^[2] Univ Estadual Paulista UNESP, Sch Dent Aracatuba, Oral Oncol Ctr, Sao Paulo - Brazil

^[3] Univ Estadual Paulista UNESP, Sch Dent Aracatuba, Dept Pathol & Clin Propedeut, Sao Paulo - Brazil

^[4] Univ Estadual Paulista UNESP, Sch Dent Aracatuba, Dept Basic Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TUMOR BIOLOGY; v. 34, n. 1, p. 139-143, FEB 2013.
• Citações Web of Science: 5

Resumo

The aim of this study was to investigate the expression of vascular endothelial growth factor type C (VEGF-C) in oral squamous cell carcinoma (OSCC) cell lines through norepinephrine-induced activation of beta-adrenergic receptors. Human OSCC cell lines (SCC-9 and SCC-25) expressing beta-adrenergic receptors were stimulated with different concentrations of norepinephrine (0.1, 1, and 10 mu M) and 1 mu M of propranolol, and analyzed after 1, 6, and 24 h. VEGF-C gene expression and VEGF-C production in the cell supernatant were evaluated by real-time PCR and by ELISA, respectively. The results showed that beta-adrenergic receptor stimulation by different concentrations of norepinephrine or blocking by propranolol did not markedly alter VEGF-C expression by SCC-9 and SCC-25 cells. VEGF-C protein levels produced by oral malignant cell lines after stimulation with different norepinephrine concentrations or blocking with propranolol was statistically similar (p > 0.05) to those of the control group (nonstimulated OSCC cell lines). Our findings suggest that stimulation of beta-adrenergic receptors by means of norepinephrine does not seem to modulate the VEGF-C expression in OSCC cell lines. These findings reinforce the need for further studies in order to understand the responsiveness of oral cancer to beta-adrenergic receptor stimulation or blockage, especially with regard to VEGF-C production. (AU)

• Processo FAPESP: 10/06333-3 - Modulação da expressão de VEGF-C por mediador relacionado ao estresse em cultura de carcinoma espinocelular de boca
• Beneficiário: Denise Tostes Oliveira
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/51361-5 - Análise dos efeitos do estresse crônico e da regulação neuroendócrina sobre o câncer de boca
• Beneficiário: Daniel Galera Bernabé
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado