| Full text | |
| Author(s): |
Rodrigues Vilardi, Bruna Maria
[1]
;
Bravo-Calderon, Diego Mauricio
[1]
;
Bernabe, Daniel Galera
[2, 3]
;
Penha Oliveira, Sandra Helena
[4]
;
Oliveira, Denise Tostes
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Sao Paulo, Bauru Sch Dent, Dept Stomatol, Area Pathol, BR-17012901 Sao Paulo - Brazil
[2] Univ Estadual Paulista UNESP, Sch Dent Aracatuba, Oral Oncol Ctr, Sao Paulo - Brazil
[3] Univ Estadual Paulista UNESP, Sch Dent Aracatuba, Dept Pathol & Clin Propedeut, Sao Paulo - Brazil
[4] Univ Estadual Paulista UNESP, Sch Dent Aracatuba, Dept Basic Sci, Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | TUMOR BIOLOGY; v. 34, n. 1, p. 139-143, FEB 2013. |
| Web of Science Citations: | 5 |
| Abstract | |
The aim of this study was to investigate the expression of vascular endothelial growth factor type C (VEGF-C) in oral squamous cell carcinoma (OSCC) cell lines through norepinephrine-induced activation of beta-adrenergic receptors. Human OSCC cell lines (SCC-9 and SCC-25) expressing beta-adrenergic receptors were stimulated with different concentrations of norepinephrine (0.1, 1, and 10 mu M) and 1 mu M of propranolol, and analyzed after 1, 6, and 24 h. VEGF-C gene expression and VEGF-C production in the cell supernatant were evaluated by real-time PCR and by ELISA, respectively. The results showed that beta-adrenergic receptor stimulation by different concentrations of norepinephrine or blocking by propranolol did not markedly alter VEGF-C expression by SCC-9 and SCC-25 cells. VEGF-C protein levels produced by oral malignant cell lines after stimulation with different norepinephrine concentrations or blocking with propranolol was statistically similar (p > 0.05) to those of the control group (nonstimulated OSCC cell lines). Our findings suggest that stimulation of beta-adrenergic receptors by means of norepinephrine does not seem to modulate the VEGF-C expression in OSCC cell lines. These findings reinforce the need for further studies in order to understand the responsiveness of oral cancer to beta-adrenergic receptor stimulation or blockage, especially with regard to VEGF-C production. (AU) | |
| FAPESP's process: | 10/51361-5 - Analise dos efeitos do estresse cronico e da regulacao neuroendocrina sobre o cancer de boca. |
| Grantee: | Daniel Galera Bernabé |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 10/06333-3 - Modulation of VEGF-C expression by stress related mediator in oral squamous cell carcinoma cell lines |
| Grantee: | Denise Tostes Oliveira |
| Support Opportunities: | Regular Research Grants |