Effect of estrogen therapy on vascular perlecan and metalloproteinases 2 and 9 in castrated rats

Autor(es)

Pompei, L. M. ^[1] ; Steiner, M. L. ^[1] ; Theodoro, T. R. ^[2] ; Souza, P. Z. ; Romanini, A. C. A. ; Coulson-Thomas, V. ^[3] ; Pinhal, M. A. S. ^[2] ; Fernandes, C. E. ^[1]

Afiliação do(s) autor(es)

^[1] Fac Med ABC, Dept Gynecol & Obstet, Santo Andre - Brazil

^[2] Fac Med ABC, Dept Biochem, Santo Andre - Brazil

^[3] Univ Fed Sao Paulo, Dept Biochem, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CLIMACTERIC; v. 16, n. 1, p. 147-153, FEB 2013.
• Citações Web of Science: 8

Resumo

Aim To study the effects of estrogen therapy on the expression of matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9) and perlecan in the vascular wall. Methods Twenty 180-day-old Wistar rats were castrated and treated 1 week later for a period of 4 weeks with one of the following: (1) placebo; (2) 0.5 mu g/day estradiol benzoate (E2B); (3) 5 mu g/day E2B; (4) 50 mu g/day E2B. A fifth group consisted of rats that had not been castrated. Following treatment, expression of MMP-2 and MMP-9 mRNA (MMP-2({[}RNA]) and MMP-9({[}RNA]), respectively) was analyzed by real-time PCR, and expression of MMP-2 (MMP-2({[}IH])), MMP-9 (MMP-9({[}IH])) and perlecan was quantified by immunohistochemistry, in carotid walls. Results There were no differences among castrated groups for MMP-2({[}RNA]) (p = 0.1969) and for MMP-9({[}RNA]) (p = 0.1828); however, a correlation was observed between E 2 B dose and MMP-9({[}RNA]) levels (r = 0.471, p = 0.018). Differences among groups were observed for MMP-2({[}IH]), MMP-9({[}IH]) and perlecan (p < 0.0001), wherein higher levels were observed in animals treated with estrogen therapy, correlating with E2B doses in the case of MMP-9 (r = 0.441, p = 0.026) and perlecan (r = 0.574, p = 0.005). Conclusions Estrogen therapy correlates with higher levels of MMP-2, MMP-9 and perlecan in the extracellular matrix of carotid walls in castrated rats, in a dose-dependent manner. There was a dose-response effect of E2B on the expression of MMP-9 mRNA and, possibly, MMP-2 mRNA. (AU)

• Processo FAPESP: 09/11382-6 - Efeito da estrogenioterapia na presença vascular de metaloproteinases do tipo 2 e 9 e inibidor tecidual de metaloproteinases do tipo 1 em ratas castradas
• Beneficiário: Luciano de Melo Pompei
• Modalidade de apoio: Auxílio à Pesquisa - Regular