Journal of Liquid Chromatography & Related Technologies;
JAN 1 2013.
Citações Web of Science:
LC-MS/MS data of the flavones from extracts of nonmodified and transgenic sugarcane (Bowman-Birk and Kunitz) led to the proposition of characteristic fragmentations that can be applied to identify the monosaccharides glucose, arabinose, and rhamnose in C-glycosylflavones. Collision-induced dissociation (CID) MS/MS generated diagnostic ions for different linked sugars in C-glycosylflavoneO-glycoside and C-glycosylflavoneO-acid derivatives, and the combination of MS data with postcolumn derivatization using UV shift reagents (which provide complementary information to determine the substitution patterns in the flavone skeleton) allowed for the identification of seven flavones not previously reported in sugarcane: 6-methoxyluteolin-8-C-arabinosyl-7-O-glucoside; diosmetin-8-C-arabinosyl-7-O-arabinoside; vitexin-7-O-rhamnoside; diosmetin-8-C-rhamnosyl-7-O-glucoside; tricetin-8C-(6?-O-sinapoylglucoside); 7,4' di-O-methylapigenin-8-C-arabinosyl-rhamnoside; and luteolin-8-C-rhamnosyl-7-O-rhamnoside. (AU)