| Full text | |
| Author(s): |
Colombo, Renata
[1]
;
Yariwake, Janete Harumi
[1]
;
Queiroz, Emerson Ferreira
[2]
;
Ndjoko, Karine
[2]
;
Hostettmann, Kurt
[2]
Total Authors: 5
|
| Affiliation: | [1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil
[2] Univ Geneva, Lab Pharmacognosie & Phytochim, Ecole Pharm Geneve Lausanne, Geneva - Switzerland
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Journal of Liquid Chromatography & Related Technologies; v. 36, n. 2, p. 239-248, JAN 1 2013. |
| Web of Science Citations: | 4 |
| Abstract | |
LC-MS/MS data of the flavones from extracts of nonmodified and transgenic sugarcane (Bowman-Birk and Kunitz) led to the proposition of characteristic fragmentations that can be applied to identify the monosaccharides glucose, arabinose, and rhamnose in C-glycosylflavones. Collision-induced dissociation (CID) MS/MS generated diagnostic ions for different linked sugars in C-glycosylflavoneO-glycoside and C-glycosylflavoneO-acid derivatives, and the combination of MS data with postcolumn derivatization using UV shift reagents (which provide complementary information to determine the substitution patterns in the flavone skeleton) allowed for the identification of seven flavones not previously reported in sugarcane: 6-methoxyluteolin-8-C-arabinosyl-7-O-glucoside; diosmetin-8-C-arabinosyl-7-O-arabinoside; vitexin-7-O-rhamnoside; diosmetin-8-C-rhamnosyl-7-O-glucoside; tricetin-8C-(6?-O-sinapoylglucoside); 7,4' di-O-methylapigenin-8-C-arabinosyl-rhamnoside; and luteolin-8-C-rhamnosyl-7-O-rhamnoside. (AU) | |