Effect of an acrylic resin combined with an antimicrobial polymer on biofilm formation

Autor(es)

Marra, Julie ^[1] ; Paleari, Andre Gustavo ^[2] ; Rodriguez, Larissa Santana ^[2] ; Perin Leite, Andressa Rosa ^[2] ; Pero, Ana Carolina ^[2] ; Compagnoni, Marco Antonio ^[2]

Afiliação do(s) autor(es)

^[1] Univ Paulista, Dept Prosthodont, Goiania, Go - Brazil

^[2] Univ Estadual Paulista UNESP, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Oral Science; v. 20, n. 6, p. 643-648, NOV-DEC 2012.
• Citações Web of Science: 13

Resumo

OBJECTIVES: The purpose of this study was to evaluate the antimicrobial activity of an acrylic resin combined with an antimicrobial polymer poly (2-tert-butylaminoethyl) methacrylate (PTBAEMA) to inhibit Staphylococcus aureus, Streptococcus mutans and Candida albicans biofilm formation. MATERIAL AND METHODS: Discs of a heat-polymerized acrylic resin were produced and divided according to PTBAEMA concentration: 0 (control), 10 and 25%. The specimens were inoculated (10(7) CFU/mL) and incubated at 37ºC for 48 h. After incubation, the wells were washed and each specimen was sonicated for 20 min. Replicate aliquots of resultant suspensions were plated at dilutions at 37ºC for 48 h. The number of colony-forming units (CFU) was counted and expressed as log (CFU+1)/mL and analyzed statistically with α=.05. RESULTS: The results showed that 25% PTBAEMA completely inhibited S. aureus and S. mutans biofilm formation. A significant reduction of log (CFU+1)/mL in count of S. aureus (control: 7.9±0.8A; 10%: 3.8±3.3B) and S. mutans (control: 7.5±0.7A; 10%: 5.1±2.7B) was observed for the group containing 10% PTBAEMA (Mann-Whitney, p<0.05). For C. albicans, differences were not significant among the groups (control: 6.6±0.2A; 10%: 6.6±0.4A; 25%: 6.4±0.1A), (Kruskal-Wallis, p>0.05, P=0.079). CONCLUSIONS: Acrylic resin combined with 10 and 25% of PTBAEMA showed significant antimicrobial activity against S. aureus and S. mutans biofilm, but it was inactive against the C. albicans biofilm. (AU)

• Processo FAPESP: 07/04061-3 - Avaliação in vitro da atividade antimicrobiana de uma resina acrílica para base protética combinada a um metacrilato
• Beneficiário: Juliê Marra
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/02602-0 - Determinação da atividade antimicrobiana de uma resina acrílica para base protética combinada a um metacrilato.
• Beneficiário: Marco Antonio Compagnoni
• Modalidade de apoio: Auxílio à Pesquisa - Regular