Quantification of B cells and T lymphocyte subsets in bovine leukemia virus infected dairy cows

Autor(es)

Melville Paiva Della Libera, Alice Maria ^[1] ; Blagitz, Maiara Garcia ^[2] ; Batista, Camila Freitas ^[3] ; Latorre, Andreia Oliveira ^[4] ; Stricagnolo, Claudia Regina ^[3] ; de Souza, Fernando Nogueira ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Clin Med, Fac Med Vet & Zootecnia, Sao Paulo - Brazil

^[2] Univ Fed Parana, UFPR, Fac Med Vet, Palotina, PR - Brazil

^[3] Univ Sao Paulo, Dept Clin Med, Fac Med Vet & Zootecnia, BR-09500900 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Dept Patol, Fac Med Vet & Zootecnia, BR-09500900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SEMINA-CIENCIAS AGRARIAS; v. 33, n. 4, p. 1487-1494, 2012.
• Citações Web of Science: 7

Resumo

The aim of the present trial was to determine the frequencies and absolute number of B and T lymphocytes subpopulations in bovine leukemia virus (BLV)-infected dairy cows with distinct lymphocyte profile known as non-leukemic (AL) and persistent lymphocytosis (PL). Thus, 15 animals were selected and divided uniformly in three groups (negative, AL, PL). The BLV infection was detected by agar gel immunodiffusion and enzyme-linked immunosorbent-assay. The lymphocytes subsets were evaluated using monoclonal antibodies by flow cytometry. The results of the present study pointed out to an increase in B lymphocytes, and also an augment in CD5(+) and CD11b(+) cells in animals showing PL. Consequently, it can be observed a decrease in the percentage of T cells subsets in these animals. Conversely, no significant alterations in the absolute number of the T lymphocytes, T CD4(+) cells and T CD8(+) lymphocytes were found in BLV-infected dairy cows with PL. Therefore, the correlation between the absolute numbers of B- and T cell subsets in the peripheral blood applied to each group showed a significant and positive strong correlation between numbers of B cells and T cells or T CD8(+) cells in the PL animals, although the same cannot be predicted for T CD4(+) lymphocytes. No such correlation was encountered for the AL and negative-control animals. (AU)

• Processo FAPESP: 07/56069-8 - Avaliacao do efeito do estresse oxidativo na resposta imune de bovinos infectados pelo virus da leucose enzootica bovina.
• Beneficiário: Alice Maria Melville Paiva Della Libera
• Modalidade de apoio: Auxílio à Pesquisa - Regular