Evaluation of the cytocompatibility of mixed bovine bone

Autor(es)

Esther Rieko Takamori ^[1] ; Eduardo Aleixo Figueira ^[2] ; Rumio Taga ^[3] ; Mari Cleide Sogayar ; José Mauro Granjeiro ^[5]

Afiliação do(s) autor(es)

^[1] University of São Paulo. School of Dentistry of Bauru. Department of Biological Sciences - Brasil

^[2] University of São Paulo. School of Dentistry of Bauru. Department of Biological Sciences - Brasil

^[3] University of São Paulo. School of Dentistry of Bauru. Department of Biological Sciences - Brasil

^[5] University of São Paulo. Cell and Molecular Therapy Center - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 18, n. 3, p. 179-184, 2007-00-00.

Resumo

Treatment of bovine bone with peroxides and chaotropic agents aims to obtain an acellular bone matrix that is able to maintain the collagen-apatite complex and a higher mechanical resistance, a mixed biomaterial hereby named mixed bovine bone (MBB). The purpose of this study was to evaluate the cytocompatibility of MBB and cell-MBB interaction. Cell morphology, number of viable cells, ability to reduce methyltetrazolium and to incorporate neutral red upon exposure to different concentrations of the hydrosoluble extract of MBB were assessed in Balb-c 3T3 cells according to ISO 10993-5 standard. The interaction between cells and MBB surface was evaluated by scanning electron microscopy. The water-soluble MBB extracts were cytotoxic and led to cell death possibly due to its effect on mitochondrial function and membrane permeability. Cells plated directly onto the MBB did not survive, although after dialysis and material conditioning in DMEM + 10% FCS, the cells adhered and proliferated onto the material. It may be concluded that, in vitro, water-soluble MBB extracts were cytotoxic. Nevertheless, MBB cytotoxic effect was reverted by dialysis resulting in a material that is suitable for cell based-therapy in the bioengineering field. (AU)

• Processo FAPESP: 01/10707-7 - Bases moleculares do controle da proliferação celular e origem de neoplasias na era de genômica e proteômica
• Beneficiário: Mari Cleide Sogayar
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 99/06460-4 - Microencapsulamento de ilhotas pancreáticas humanas e engenharia de tecidos como alternativa terapêutica do Diabetes mellitus
• Beneficiário: Mari Cleide Sogayar
• Modalidade de apoio: Auxílio à Pesquisa - Parceria para Inovação Tecnológica - PITE