Efficacy of an enzyme-linked immunosorbent assay as a diagnostic tool for schistosomiasis mansoni in individuals with low worm burden

Autor(es)

Edward José de Oliveira ^[1] ; Hermínia Yohko Kanamura ^[2] ; Dirce Mary Correia Lima ^[3]

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas - Brasil

^[2] Universidade de Taubaté. Departamento de Biologia - Brasil

^[3] Instituto de Medicina Tropical de São Paulo. Laboratório de Investigação Médica (LIM06) - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Memórias do Instituto Oswaldo Cruz; v. 100, n. 4, p. 421-425, 2005-07-00.

Resumo

IgM-ELISA is an immunoenzymatic method useful for detection of IgM antibodies against a fraction of Schistosoma mansoni adult worm antigen (AWA) that is soluble in trichloroacetic acid (AWA-TCA). This method was applied to three groups of individuals with different clinical and epidemiological characteristics, and the results compared with those obtained by other diagnostic methods: immunofluorescence test for detection of IgM antibodies (IgM-IFT) or IgG antibodies (IgG-IFT), ELISA for detection of IgG antibodies (IgG-ELISA), and two parasitological methods, Kato-Katz and miracidium hatching. The IgM-ELISA presented a sensitivity of 98%, when the parasitologic fecal examination was defined as reference diagnostic method, and a specificity of 98 and 97.3%, respectively for the group of clinically healthy individuals and other helminth carriers. A comparative analysis between the results of IgM-ELISA and those obtained by other serologic tests showed a good degree of agreement, with Kappa indices ranging from 0.95 to 0.98. The diagnostic efficacy of 97.8%, as determined with schistosomiasis patients with low parasitic burden, suggests the excellent performance of the IgM-ELISA and its usefulness for the diagnosis of schistosomiasis when applied in low endemic areas. (AU)

• Processo FAPESP: 97/13904-0 - Caracterizacao das proteinas de 31-32 kda e da hsp70 do schistosoma mansoni e producao de imunoreagentes de interesse diagnostico para a esquistossomose mansonica.
• Beneficiário: Herminia Yohko Kanamura
• Modalidade de apoio: Auxílio à Pesquisa - Regular