Molecular characterization of astrovirus in stool samples from children in São Paulo, Brazil

Autor(es)

Hugo Reis Resque ^[1] ; Veridiana Munford ^[2] ; Juliana Galera Castilho ^[3] ; Herika Schmich ^[4] ; Thabata Alessandra Ramos Caruzo ^[5] ; Maria Lucia Rácz ^[6]

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. Instituto de Ciências Biomédicas. Laboratório de Gastroenterites Virais - Brasil

^[2] Universidade de São Paulo. Instituto de Ciências Biomédicas. Laboratório de Gastroenterites Virais - Brasil

^[3] Instituto Pasteur. Seção de Diagnóstico - Brasil

^[4] Faculdade de Medicina do ABC. CEPES - Brasil

^[5] Becton Dickinson Indústrias Cirúrgicas Ltda. Diagnostic Systems - Brasil

^[6] Universidade de São Paulo. Instituto de Ciências Biomédicas. Laboratório de Gastroenterites Virais - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Memórias do Instituto Oswaldo Cruz; v. 102, n. 8, p. 969-974, 2007-12-00.

Resumo

The purpose of this study was to characterize astrovirus in faecal samples collected from children with and without diarrhea in São Paulo, Brazil, grouped into two sets: EPM and HU. Detection and genotyping were carried out using reverse transcription nested polymerase chain reaction (RT-PCR) with specific primers directed towards the genome open reading frame 2 (ORF2). Results for EPM set showed that 66/234 (28.2%) were positive: 28/94 (29.7%) from children with acute diarrhea, 14/45 (31.1%) with persistent diarrhea, and 9/55 (16.3%) from control individuals. No data was available for 15/40 (37.5%) of samples. Mixed infections with other viruses were found in 33 samples. In the HU, 18/187 (9.6%) were positive: 12/158 (7.6%) from individuals with acute diarrhea and 6/29 (20.7%) from control children. Four samples were mixed with other viruses. Out of 66 astrovirus positive EPM samples, 18 (27.2%) were characterized as human astrovirus type-1 (HAstV-1), two (3.0%) as HAstV-2, two (3.0%) as HAstV-3, and three (4.5%) as HAstV-8. Among 18 astrovirus positive HU samples, one (5.5%) was characterized as HAstV-1, six (33.3%) as HAstV-2, and one (5.5%) as HAstV-8. Two HAstV-8 genotyped samples were further confirmed by nucleotide sequencing. Our results shows that astroviruses are circulating in a constant manner in the population, with multiple serotypes, in higher frequency than it was described for other Brazilian regions. For the first time in Sao Paulo, Brazil, it was shown that astroviruses play an important role in children gastroenteritis, as described for most locations where they were detected. (AU)

• Processo FAPESP: 01/14335-7 - Caracterização molecular de astrovírus em amostras fecais de crianças com gastroenterite em São Paulo, Brasil
• Beneficiário: HUGO REIS RESQUE
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 00/14461-0 - Sorotipagem e caracterização molecular de genótipos G e P de amostras de rotavírus de humanos
• Beneficiário: Herika Schmich
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 01/07298-8 - Identificação, genotipagem e sequenciamento genômico de vírus Norwalk-like em amostras fecais de crianças com gastroenterite
• Beneficiário: Maria Lucia Barbosa de Oliveira Racz
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 00/11857-0 - Sequenciamento genômico de amostras atípicas de rotavírus bovino do estado de Goiás
• Beneficiário: Thabata Alessandra Ramos Caruzo
• Modalidade de apoio: Bolsas no Brasil - Mestrado