Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Insensitive trypsins are differentially transcribed during Spodoptera frugiperda adaptation against plant protease inhibitors

Texto completo
Autor(es):
Ramalho de Oliveira, Caio Fernando [1, 2] ; Souza, Thais de Paula [3] ; Postali Parra, Jose Roberto [4] ; Marangoni, Sergio [1] ; Silva-Filho, Marcio C. [3] ; Rodrigues Macedo, Maria Ligia [2, 3]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Inst Biol, Dept Biochem, BR-13083970 Campinas, SP - Brazil
[2] Univ Mato Grosso Sul, Ctr Biol & Hlth Sci, Dept Food Technol & Publ Hlth, BR-79070900 Campo Grande, MS - Brazil
[3] Univ Sao Paulo, Dept Genet, Piracicaba, SP - Brazil
[4] Univ Sao Paulo, Dept Entomol, Piracicaba, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY; v. 165, n. 1, p. 19-25, MAY 2013.
Citações Web of Science: 25
Resumo

The fall armyworm (Spodoptera frugiperda) is an important pest insect due to high degree of polyphagia. In order to better understand its adaptation mechanism against plant protease inhibitors, bioassays were carried supplementing diet with the Kunitz trypsin inhibitor from Entada acaciifolia seeds (EATI). In vitro assays showed an increase of proteolytic activity in EATI-fed larvae midgut Moreover, the trypsin enzymes showed insensitivity to inhibition with EATI. In order to understand what genes were overexpressed after chronic exposition to EATI, quantitative RT-PCR analyses were performed and revealed an increase in transcription of two trypsin genes, suggesting its participation in insensitivity of midgut trypsins. Another important result was the expression of one chymotrypsin gene, which is not expressed in control fed-larvae but induced in EATI-fed larvae. New regions of higher molecular weight showing proteolytic activity were visualized in inhibitor-fed larvae by zymography gel electrophoresis, proposing that the new enzymes expressed in response of inhibitor dietary would be formatting oligomers. This is a characteristic also observed in other pest insects that adapt to feed in plant protease inhibitors diet. Additional assays revealed that trypsins from EATI-fed larvae also became insensitive against Kunitz and Bowman-Birk inhibitors from soybean. This result suggests a possible involvement of the same S. frugiperda genes in adaptation against Kunitz and Bowman-Birk inhibitors in their host plants. (C) 2013 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 11/09361-0 - Inibidor de tripsina de Entada acaciifolia (EATI): caracterização estrutural e avaliação do potencial inseticida contra a lagarta-do-cartucho (Spodoptera frugiperda)
Beneficiário:Sergio Marangoni
Linha de fomento: Auxílio à Pesquisa - Regular