Development of an analytical method for the quantification of pfaffic acid in Brazilian ginseng (Hebanthe eriantha)

Autor(es)

Nova Rodrigues, Marili Villa ^[1] ; Souza, Kelly de Paula ^[2] ; Garcia Rehder, Vera Lucia ^[1] ; Vilela, Gisele Freitas ^[3] ; Montanari Junior, Ilio ^[1] ; Figueira, Glyn Mara ^[1] ; Rath, Susanne ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Chem Biol & Agr Res Ctr CPQBA, BR-13083970 Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Dept Analyt Chem, BR-13084971 Campinas, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Fac Agr Sci, BR-18610307 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Pharmaceutical and Biomedical Analysis; v. 77, p. 76-82, APR 15 2013.
• Citações Web of Science: 1

Resumo

Hebanthe eriantha (Poir.) Pedersen (Amaranthaceae), which is known as Brazilian ginseng is widely used in folk medicine as an aphrodisiac and antidiabetic tonic. The anti-tumor activity, attributed to the pfaffic acid present in roots of H. eriantha, is responsible for the great interest in the commercialization of this species. In Brazil, the species H. eriantha is mainly used in commercial preparations, although other plants of the genus Pfaffia and Hebanthe have been marketed as Pfaffia paniculata or Brazilian ginseng. The pfaffic acid present in the roots is mainly conjugated with sugars (pfaffosides) and can be used as an active marker of H. eriantha, which helps to differentiate this species from others marketed as Brazilian ginseng. The main objective of this study was to develop and validate a liquid chromatographic method to quantify pfaffic acid in the roots of H. eriantha. The extraction and hydrolysis conditions were optimized using an univariate and experimental design, respectively, and the quantification of pfaffic acid by high performance liquid chromatography with diode-array detection (HPLC-DAD) was validated. This method was used to evaluate the pfaffic acid content in 30 different genotypes of the species from a germplasm collection. The content of pfaffic acid ranged from 0.97 to 4.29% (w/w) on a dry weight basis. (c) 2013 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 08/01376-6 - Desenvolvimento analítico para quantificação de ácido pfáffico em genótipos de Pfaffia paniculata a partir da obtenção do padrão analítico
• Beneficiário: Marili Villa Nova Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Regular