Sugarcane bagasse ozonolysis pretreatment: Effect on enzymatic digestibility and inhibitory compound formation

Autor(es)

Travaini, Rodolfo ^[1] ; Morales Otero, Marian Derly ^[2] ; Coca, Monica ^[1] ; Da-Silva, Roberto ^[3] ; Bolado, Silvia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Valladolid, Dept Chem Engn & Environm Technol, E-47011 Valladolid - Spain

^[2] Univ Valle, Sch Environm & Nat Resources Engn, Lab Environm Microbiol & Biotechnol, Santiago De Cali 25360 - Colombia

^[3] Sao Paulo State Univ, UNESP, IBILCE, Lab Biochem & Appl Microbiol, Dept Chem & Environm, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Bioresource Technology; v. 133, p. 332-339, APR 2013.
• Citações Web of Science: 58
• Assunto(s): Bagaço de cana-de-açúcar Etanol Bioetanol

Resumo

Sugarcane bagasse was pretreated with ozone to increase lignocellulosic material digestibility. Bagasse was ozonated in a fixed bed reactor at room temperature, and the effect of the two major parameters, ozone concentration and sample moisture, was studied. Acid insoluble and total lignin decreased whereas acid soluble lignin increased in all experiments. Pretreatment barely attacked carbohydrates, with cellulose and xylan recovery rates being >92%. Ozonolysis increased fermentable carbohydrate release considerably during enzymatic hydrolysis. Glucose and xylose yields increased from 6.64% and 2.05%, for raw bagasse, to 41.79% and 52.44% under the best experimental conditions. Only xylitol, lactic, formic and acetic acid degradation compounds were found, with neither furfural nor HMF (5-hydroxymethylfurfural) being detected. Washing detoxification provided inhibitor removal percentages above 85%, increasing glucose hydrolysis, but decreasing xylose yield by xylan solubilization. SEM analysis showed structural changes after ozonization and washing. (C) 2013 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 07/08641-4 - Estudo da produção e comparação da ação de celulases e hemicelulases de diferentes fungos termofílicos e mesofílicos sobre a sacarificação de bagaço de cana-de-açúcar
• Beneficiário: Rodolfo Travaini
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 10/12624-0 - Aplicação de métodos físico-químicos e enzimáticos na sacarificação do bagaço de cana: estudos de microrganismos, processos fermentativos relacionados e métodos de hidrólise
• Beneficiário: Eleni Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Temático