Assessment of the Genotoxicity of Two Agricultural Residues After Processing by Diplopods Using the Allium cepa Assay

Autor(es)

Christofoletti, Cintya A. ^[1] ; Pedro-Escher, Janaina ^[1] ; Fontanetti, Carmem S. ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Sao Paulo State Univ, BR-13506900 Rio Claro, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: WATER AIR AND SOIL POLLUTION; v. 224, n. 4 APR 2013.
• Citações Web of Science: 16

Resumo

Agroindustrial by-products and residues from treatment of sewage sludge have been recently recycled as soil amendments. This study was aimed at assessing toxic potential of biosolid, obtained from a sewage treatment plant (STP), vinasse, a by-product of the sugar cane industry, and a combination of both residues using Allium cepa assay. Bioprocessing of these samples by a terrestrial invertebrate (diplopod Rhinocricus padbergi) was also examined. Bioassay assembly followed standards of the Brazilian legislation for disposal of these residues. After adding residues, 20 diplopods were placed in each terrarium, where they remained for 30 days. Chemical analysis and the A. cepa assay were conducted before and after bioprocessing by diplopods. At the end of the bioassay, there was a decrease in arsenic and mercury. For the remaining metals, accumulation and/or bioavailability varied in all samples but suggested bioprocessing by animals. The A. cepa test revealed genotoxic effects characterized by different chromosome aberrations. Micronuclei and chromosome breaks on meristematic cells and F-1 cells with micronuclei were examined to assess mutagenicity of samples. After 30 days, the genotoxic effects were significantly reduced in the soil+biosolid and soil+biosolid+vinasse groups as well as the mutagenic effects in the soil+biosolid+vinasse group. Similar to vermicomposting, bioprocessing of residues by diplopods can be a feasible alternative and used prior to application in crops to improve degraded soils and/or city dumps. Based on our findings, further studies are needed to adequately dispose of these residues in the environment. (AU)

• Processo FAPESP: 09/53047-9 - Analise do potencial toxico da vinhaca utilizando diferentes organismos teste
• Beneficiário: Janaína Pedro Escher
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/50578-3 - Avaliacao da toxicidade de residuos industriais e urbanos aplicados na agricultura.
• Beneficiário: Cintya Aparecida Christofoletti de Figueiredo
• Modalidade de apoio: Bolsas no Brasil - Doutorado