First isolation and genotyping of Toxoplasma gondii from bats (Mammalia: Chiroptera)

Autor(es)

Cabral, A. D. ^[1] ; Gama, A. R. ^[2] ; Sodre, M. M. ^[2] ; Savani, E. S. M. M. ^[2] ; Galvao-Dias, M. A. ^[2] ; Jordao, L. R. ^[2] ; Maeda, M. M. ^[2] ; Yai, L. E. O. ^[2] ; Gennari, S. M. ^[1] ; Pena, H. F. J. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, BR-05508270 Sao Paulo - Brazil

^[2] CCZ, Lab Zoonoses & Doencas Transmitidas Vetores LabZo, BR-02031020 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Veterinary Parasitology; v. 193, n. 1-3, p. 100-104, MAR 31 2013.
• Citações Web of Science: 19

Resumo

There are currently no reports on the isolation and molecular examination of Toxoplasma gondii from bats. Here, we report the isolation and genotypic characterisation of two T. gondii isolates from bats. A total of 369 bats from different municipalities in Sao Paulo state, southeastern Brazil, were captured and euthanised, and collected tissues (heart and pectoral muscle) were processed for each bat or in pools of two or three bats and bioassayed in mice (a total of 283 bioassays). Eleven PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) markers were used to genotype positive samples: SAG1, SAG2 (5'-3'SAG2 and alt. SAG2), SAG3,BTUB, GRA6, L358, c22-8, c29-2, PK1, CS3 and Apico. The parasite was isolated from two bats from Sao Paulo city: an insectivorous bat, the velvety free-tailed bat Molossus molossus, and a hematophagous bat, the common vampire bat Desmodus rotundus. Isolates were designated TgBatBr1 and TgBatBr2, respectively. The genotype of the isolate from M. molossus (TgBatBr1) has been previously described in an isolate from a capybara from Sao Paulo state, and the genotype from the D. rotundus isolate (TgBatBr2) has already been identified in isolates from cats, chickens, capybaras, sheep, a rodent and a common rabbit from different Brazilian states, suggesting that this may be a common T. gondii lineage circulating in some Brazilian regions. Isolation of T. gondii from a hematophagous species is striking. This study reveals that bats can share the same isolates that are found in domesticated and wild terrestrial animals. This is the first report of the isolation and genotyping of T. gondii in chiropterans. (C) 2012 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 09/51889-2 - Epidemiologia da Toxoplasma gondii (soroprevalência, isolamento e genotipagem) e investigação molecular de outros protozoários perten centes a sub-família Toxoplasmatinae (Neospora spp e hammondia spp)..
• Beneficiário: Aline Diniz Cabral
• Modalidade de apoio: Bolsas no Brasil - Doutorado