An evaluation of chondrocyte morphology and gene expression on superhydrophilic vertically-aligned multi-walled carbon nanotube films

Autor(es)

Antonioli, Eliane ^[1] ; Lobo, Anderson O. ^[2] ; Ferretti, Mario ^{[1, 3]} ; Cohen, Moises ^{[1, 3]} ; Marciano, Fernanda R. ^[2] ; Corat, Evaldo J. ^[4] ; Trava-Airoldi, Vladimir J. ^[4]

Afiliação do(s) autor(es)

^[1] Hosp Israelita Albert Einstein, Res & Educ Inst, Sao Paulo - Brazil

^[2] Univ Vale Paraiba, Lab Biomed Nanotechnol, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Ortophed Div, Sao Paulo - Brazil

^[4] Inst Nacl Pesquisas Espaciais, Lab Associado Sensores & Mat, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 33, n. 2, p. 641-647, MAR 1 2013.
• Citações Web of Science: 13

Resumo

Cartilage serves as a low-friction and wear-resistant articulating surface in diarthrodial joints and is also important during early stages of bone remodeling. Recently, regenerative cartilage research has focused on combinations of cells paired with scaffolds. Superhydrophilic vertically aligned carbon nanotubes (VACNTs) are of particular interest in regenerative medicine. The aim of this study is to evaluate cell expansion of human articular chondrocytes on superhydrophilic VACNTs, as well as their morphology and gene expression. VACNT films were produced using a microwave plasma chamber on Ti substrates and submitted to an O-2 plasma treatment to make them superhydrophilic. Human chondrocytes were cultivated on superhydrophilic VACNTs up to five days. Quantitative RT-PCR was performed to measure type I and type II Collagen, Sox9, and Aggrecan mRNA expression levels. The morphology was analyzed by scanning electron microscopy (SEM) and confocal microscopy. SEM images demonstrated that superhydrophilic VACNTs permit cell growth and adhesion of human chondrocytes. The chondrocytes had an elongated morphology with some prolongations. Chondrocytes cultivated on superhydrophilic VACNTs maintain the level expression of Aggrecan, Sox9, and Collagen II determined by gPCR. This study was the first to indicate that superhydrophilic VACNTs may be used as an efficient scaffold for cartilage or bone repair. (C) 2012 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 11/20345-7 - Estudo de filmes de carbono-tipo diamante contendo nanopartículas incorporadas para aplicações biomédicas
• Beneficiário: Fernanda Roberta Marciano
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 11/17877-7 - Desenvolvimento de novos scaffolds poliméricos por eletrofiação com incorporação de nanotubos alinhados e nanohidroxiapatita para regeneração óssea
• Beneficiário: Anderson de Oliveira Lobo
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores