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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Phototoxic effect of curcumin on methicillin-resistant Staphylococcus aureus and L929 fibroblasts

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Autor(es):
Dias Ribeiro, Ana Paula [1] ; Pavarina, Ana Claudia [1, 2] ; Dovigo, Livia Nordi [1] ; Brunetti, Iguatemy Lourenco [3] ; Bagnato, Vanderlei Salvador [4] ; Vergani, Carlos Eduardo [1] ; de Souza Costa, Carlos Alberto [5]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] UNESP Univ Estadual Paulista, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil
[2] Univ Estadual Paulista UNESP, Fac Odontol Araraquara, BR-14801903 Sao Paulo - Brazil
[3] UNESP Univ Estadual Paulista, Dept Clin Anal, Sch Pharmaceut Sci, Araraquara, SP - Brazil
[4] Univ Sao Paulo, Phys Inst Sao Carlos, Sao Carlos, SP - Brazil
[5] UNESP Univ Estadual Paulista, Araraquara Sch Dent, Dept Physiol & Pathol, Araraquara, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Lasers in Medical Science; v. 28, n. 2, p. 391-398, FEB 2013.
Citações Web of Science: 44
Resumo

Photodynamic therapy has been investigated as an alternative method of killing pathogens in response to the multiantibiotic resistance problem. This study evaluated the photodynamic effect of curcumin on methicillin-resistant Staphylococcus aureus (MRSA) compared to susceptible S. aureus (MSSA) and L929 fibroblasts. Suspensions of MSSA and MRSA were treated with different concentrations of curcumin and exposed to light-emitting diode (LED). Serial dilutions were obtained from each sample, and colony counts were quantified. For fibroblasts, the cell viability subsequent to the curcumin-mediated photodynamic therapy was evaluated using the MTT assay and morphological changes were assessed by SEM analysis. Curcumin concentrations ranging from 5.0 to 20.0 mu M in combination with any tested LED fluences resulted in photokilling of MSSA. However, only the 20.0 mu M concentration in combination with highest fluence resulted in photokilling of MRSA. This combination also promoted an 80% reduction in fibroblast cell metabolism and morphological changes were present, indicating that cell membrane was the main target of this phototherapy. The combination of curcumin with LED light caused photokilling of both S. aureus strains and may represent an alternative treatment for eradicating MRSA, responsible for significantly higher morbidity and mortality and increased healthcare costs in institutions and hospitals. (AU)

Processo FAPESP: 08/03994-9 - Estudo da efetividade da terapia fotodinâmica antimicrobiana na inativação de espécies de Candida e do dano celular causado nas células fúngicas: avaliação in vitro e in vivo de dois fotossensibilizadores
Beneficiário:Ana Cláudia Pavarina
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 10/05425-1 - Avaliação da efetividade da terapia fotodinâmica antimicrobiana na inativação de espécies de Candida e do dano celular causado a células epiteliais: avaliação in vitro e in vivo da cloro alumínio ftalocianina e e outros derivados
Beneficiário:Ana Cláudia Pavarina
Modalidade de apoio: Auxílio à Pesquisa - Regular