Fluoride modulates preosteoblasts viability and matrix metalloproteinases-2 and -9 activities

Autor(es)

Camila Slompo ^[1] ; Camila P. Buzalaf ^[2] ; Carla A. Damante ^[3] ; Gisele M. Martins ^[4] ; Angélica R Hannas ^[5] ; Marília A. R Buzalaf ^[6] ; Rodrigo C Oliveira ^[7]

Afiliação do(s) autor(es)

^[1] University of São Paulo - Brasil

^[2] University of São Paulo - Brasil

^[3] University of São Paulo - Brasil

^[4] University of São Paulo - Brasil

^[5] University of São Paulo - Brasil

^[6] University of São Paulo - Brasil

^[7] University of São Paulo - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 23, n. 6, p. 629-634, 2012-00-00.

Resumo

This study evaluated the influence of fluoride on cell viability and activity of matrix metalloproteinases (MMP) -2 and -9 secreted by preosteoblasts. Preosteoblasts (MC3T3-E1 murine cell line) were cultured in MEM medium supplement with 10% Fetal Bovine Serum (FBS) and nucleosides/ribonucleosides without ascorbic acid. Adherent cells were treated with different concentrations of F (as sodium fluoride-NaF) in medium (5 x 10-6 M, 10-5 M, 10-4 M and 10-3 M) for 24, 48, 72 and 96 h at 37ºC, 5% CO2. Control cells were cultivated in MEM only. After each period, preosteoblast viability was assessed by MTT assay. MMP-2 and -9 activities were performed by gel zymography. Also, alkaline phosphatase (ALP) activity was quantified by colorimetry in all experimental groups. It was shown that cultured cells with the highest dose of F (10-3 M) for 96 h decreased preosteoblast viability while lower doses of F did not alter it, when compared to untreated cells. No differences were observed in ALP activity among groups. Moreover, compared to control, the treatment of cells with F at low dose slightly increased MMP-2 and -9 activities after 24 h. It was concluded that F modulates preosteoblast viability in a dose-dependent manner and also may regulate extracellular matrix remodeling. (AU)

• Processo FAPESP: 08/00322-0 - Influência do flúor na atividade de fosfatases e metaloproteinases de matriz 2 e 9, em osteoblastos.
• Beneficiário: Camila Slompo
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica