FAS and FAS-L Genotype and Expression in Patients With Recurrent Pregnancy Loss

Autor(es)

Andrade Banzato, Priscilla Chamelete ^[1] ; Daher, Silvia ^[1] ; Traina, Evelyn ^[1] ; Torloni, Maria Regina ^[1] ; Gueuvoghlanian-Silva, Barbara Yasmin ^[1] ; Puccini, Renata Fiorini ^[1] ; Tezotto Pendeloski, Karen Priscilla ^[1] ; Mattar, Rosiane ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Obstet, BR-04037001 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: REPRODUCTIVE SCIENCES; v. 20, n. 9, p. 1111-1115, SEP 2013.
• Citações Web of Science: 9

Resumo

We assessed FAS and FAS-L gene polymorphisms and messenger RNA (mRNA) levels in patients with recurrent pregnancy loss (RPL). This case-control study compared 129 women with RPL with 235 healthy multiparous women (control group). Genomic DNA and total mRNA were extracted from whole blood, and polymorphisms genotyping was performed by polymerase chain reaction (PCR). Messenger RNA expression levels were analyzed by real-time PCR. Data were analyzed by chi-square and Fisher exact tests; P < .05 was considered significant. There were no significant differences in the FAS (670 A/G) genotype or allelic frequencies between the RPL and control groups. We found significant differences in the FAS-L (844 C/T) genotype and allelic frequencies between women with RPL and controls. Patients with RPL had significantly higher FAS-L expression. Our data suggest that FAS-L gene polymorphism is associated with increased susceptibility to RPL. Moreover, women with RPL seem to abnormally express FAS-FAS-L molecules. (AU)

• Processo FAPESP: 09/50236-5 - Avaliação da relação entre polimorfismos de genes codificadores de FAS e Fas-L e o aborto espontâneo de repetição
• Beneficiário: Rosiane Mattar
• Modalidade de apoio: Auxílio à Pesquisa - Regular