Species diversity of culturable endophytic fungi from Brazilian mangrove forests

Autor(es)

de Souza Sebastianes, Fernanda Luiza ^[1] ; Romao-Dumaresq, Aline Silva ^[1] ; Lacava, Paulo Teixeira ^[2] ; Harakava, Ricardo ^[3] ; Azevedo, Joao Lucio ^[1] ; de Melo, Itamar Soares ^[4] ; Pizzirani-Kleiner, Aline Aparecida ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Genet, BR-13400970 Piracicaba, SP - Brazil

^[2] Univ Fed Sao Carlos, Ctr Biol Sci & Hlth, BR-13565905 Sao Carlos, SP - Brazil

^[3] Inst Biol, Phytopathol & Biochem Lab, BR-04014002 Sao Paulo - Brazil

^[4] EMBRAPA, Lab Environm Microbiol, BR-13820000 Jaguariuna, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CURRENT GENETICS; v. 59, n. 3, p. 153-166, AUG 2013.
• Citações Web of Science: 24

Resumo

This study aimed to perform a comparative analysis of the diversity of endophytic fungal communities isolated from the leaves and branches of Rhizophora mangle, Avicennia schaueriana and Laguncularia racemosa trees inhabiting two mangroves in the state of So Paulo, Brazil {[}Cananeia and Bertioga (oil spill-affected and unaffected)] in the summer and winter. Three hundred and forty-three fungi were identified by sequencing the ITS1-5.8S-ITS2 region of rDNA. Differences were observed in the frequencies of fungi isolated from the leaves and branches of these three different plant species sampled from the Bertioga oil spill-affected and the oil-unaffected mangrove sites in the summer and winter; these differences indicate a potential impact on fungal diversity in the study area due to the oil spill. The molecular identification of the fungi showed that the fungal community associated with these mangroves is composed of at least 34 different genera, the most frequent of which were Diaporthe, Colletotrichum, Fusarium, Trichoderma and Xylaria. The Shannon and the Chao1 indices {[}H'((95 %)) = 4.00, H'((97 %)) = 4.22, Chao1((95 %)) = 204 and Chao1((97 %)) = 603] indicated that the mangrove fungal community possesses a vast diversity and richness of endophytic fungi. The data generated in this study revealed a large reservoir of fungal genetic diversity inhabiting these Brazilian mangrove forests and highlighted substantial differences between the fungal communities associated with distinct plant tissues, plant species, impacted sites and sampling seasons. (AU)

• Processo FAPESP: 04/13910-6 - Biodiversidade e atividades funcionais de microrganismos de manguezais do estado de São Paulo
• Beneficiário: Itamar Soares de Melo
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 06/57060-1 - Análise da comunidade bacteriana associada a manguezais do estado de São Paulo com interesse biotecnológico e agrícola
• Beneficiário: Paulo Teixeira Lacava
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado