Effect of bixin and norbixin on the expression of cytochrome P450 in HepG2 cell line

Autor(es)

Sakuragui Matuo, Miriam Cristina ^[1] ; de Oliveira Takamoto, Rafael Teruiti ^[1] ; Kikuchi, Irene Satiko ^[1] ; Andreoli Pinto, Terezinha de Jesus ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Pharm, Fac Pharmaceut Sci, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Cell Biology International; v. 37, n. 8, p. 843-848, AUG 2013.
• Citações Web of Science: 7

Resumo

Bixin and norbixin are the main components of annatto, which is extracted from Bixa orellana and largely used as natural colorant in the food and pharmaceutical industries. Annatto can enhance CYP1A and CYP2B activity in rats; however, the inducer effect has not been investigated in human cell lines. In this study, the ability of bixin and norbixin to induce the cytochrome P450 (CYP) enzymes was assessed in HepG2 human hepatoma cell line. HepG2 cells were treated with bixin and norbixin, and the expression of the CYP genes quantified by real-time reverse transcription polymerase chain reaction (RT-PCR). Expression of CYP1A1 and CYP1A2 was significantly increased by bixin treatment, while CYP2B6, 2C9, 2E1 and 3A4 were unaffected. Cells were treated with norbixin showed no inducer effect. The results suggest that the inducer potential of annatto is attributed to bixin, but not to norbixin, despite their similarities in molecular structure. (AU)

• Processo FAPESP: 09/54375-0 - Linhagens de hepatoma humano induzidas por bixina como modelo in vitro no desenvolvimento de fármacos
• Beneficiário: Terezinha de Jesus Andreoli Pinto
• Modalidade de apoio: Auxílio à Pesquisa - Regular