Myocardial Infarction Area Quantification using High-Resolution SPECT Images in Rats

Autor(es)

Lemos de Oliveira, Luciano Fonseca ^[1] ; Mejia, Jorge ^[2] ; Vieira de Carvalho, Eduardo Elias ^[1] ; Lataro, Renata Maria ^[1] ; Frassetto, Sarita Nasbine ^[1] ; Fazan, Jr., Rubens ^[3] ; Salgado, Helio Cesar ^[3] ; Galvis-Alonso, Orfa Yineth ^[2] ; Simoes, Marcus Vinicius ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Clin Med, Div Cardiol, BR-14049 Ribeirao Preto, SP - Brazil

^[2] Fac Med Sao Jose do Rio Preto, Sao Jose Do Rio Preto, SP - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Fisiol, BR-14049 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Arquivos Brasileiros de Cardiologia; v. 101, n. 1, p. 59-67, JUL 2013.
• Citações Web of Science: 7

Resumo

Background: Imaging techniques enable in vivo sequential assessment of the morphology and function of animal organs in experimental models. We developed a device for high-resolution single photon emission computed tomography (SPECT) imaging to upgrade a clinical gamma-camera, based on pinhole collimator. Objective: To determine the accuracy of this system for quantification of myocardial infarct area in rats. Methods: Thirteen male Wistar rats (250 g) underwent experimental myocardial infarction by occlusion of the left coronary artery. After 4 weeks, SPECT images were acquired 1.5 hours after intravenous injection of 555 MBq of 99mTc-Sestamibi. The tomographic reconstruction was performed by using specially developed software based on the Maximum Likelihood algorithm. The analysis of the data included the correlation between the area of perfusion defects detected by scintigraphy and extent of myocardial fibrosis assessed by histology. Results: The images showed a high target organ/background ratio with adequate visualization of the left ventricular walls and cavity. All animals presenting infarction areas were correctly identified by the perfusion images. There was no difference of the infarct area as measured by SPECT (21.1 +/- 21.2%) and by histology (21.7 +/- 22.0%; p=0.45). There was a strong correlation between individual values of the area of infarction measured by these two methods. Conclusion: The developed system presented adequate spatial resolution and high accuracy for the detection and quantification of myocardial infarction areas, consisting in a low cost and versatile option for high-resolution SPECT imaging of small rodents. (AU)

• Processo FAPESP: 11/03261-4 - Estudo das alterações da inervação simpática cardíaca e da perfusão miocárdica e sua correlação com a progressão da disfunção ventricular esquerda em modelo experimental de cardiomiopatia chagásica crônica no hamster
• Beneficiário: Marcus Vinicius Simões
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/50339-3 - Desenvolvimento de instrumentação e de algoritmos de processamento de dados para a produção de imagens cintilográficas de pequenos animais com resolução espacial e temporal elevadas
• Beneficiário: Jorge Mejía Cabeza
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores