Morphological and cytochemical changes in synganglion of Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) female ticks from exposure of andiroba oil (Carapa guianensis)

Autor(es)

Roma, Gislaine Cristina ^[1] ; Camargo Mathias, Maria Izabel ^[1] ; De Faria, Adriano Uemura ^[1] ; De Oliveira, Patricia Rosa ^[1] ; Scopinho Furquim, Karim Christina ^[1] ; Bechara, Gervasio Henrique ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Inst Biociencias, Dept Biol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROSCOPY RESEARCH AND TECHNIQUE; v. 76, n. 7, p. 687-696, JUL 2013.
• Citações Web of Science: 9

Resumo

Actually, the most used method to control ticks is synthetic acaricides with neurotoxic action. However, the use of these methods presents inconveniences, such as the contamination of the environment and risks to the host's health due to the residual effects. Thus, several studies have been developed aiming to find alternative ways to control these ectoparasites, such as the use of natural compounds with active ingredients, which act controlling some species of plagues in addition to presenting medicinal properties that are beneficial to humans. The present study aimed to analyze the action of andiroba oil (Carapa guianensis) on the synganglion of Rhipicephalus sanguineus semiengorged females through morphological and cytochemical techniques aiming to verify if this natural product have neurotoxic action as the numerous synthetic acaricides. The results showed that andiroba oil interferes in the synganglion through structural and enzymatic changes, which lead the nervous tissue to apoptotic death involving autophagy. Among these changes was observed the emergence of large empty spaces between the perineurium and the cortical region, vacuolated cortex cells and with cell swelling, neural cells with picnotic nuclei or in initial stage of chromatin margination and neuropile with high structural disorganization. Considering these data, it can be concluded that andiroba seed oil can be used as an alternative method in the control of R. sanguineus ticks due to its neurotoxic action. Microsc. Res. Tech. 76:687-696, 2013. (c) 2013 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 11/10427-6 - Auxílio técnico para manutenção de colônias de carrapatos e processamento de técnicas laboratoriais
• Beneficiário: Mateus Ribeiro Mota
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 11/07938-9 - Avaliação das alterações celulares nos ovócitos de fêmeas semi-ingurgitadas de Carrapatos Rhipicephalus sanguineus (Acari: Ixodidae) decorrentes da exposição ao óleo de andiroba (Carapa guianensis)
• Beneficiário: Maria Claudia Ramalho Vendramini
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 11/06865-8 - Estudo morfofisiológico comparativo do sistema nervoso de larvas, ninfas e adultos de carrapatos Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) e avaliação celular do singânglio de fêmeas semi-ingurgitadas expostas à permetrina e ao óleo de andiroba
• Beneficiário: Gislaine Cristina Roma
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores
• Processo FAPESP: 10/51942-8 - Estudo morfofisiológico comparativo do sistema nervoso de larvas, ninfas e adultos de carrapatos Rhipicephalus sanguineus (Acari: Ixodidae) e avaliação celular do singânglio de fêmeas semi-ingurgitadas expostas à permetrina e ao óleo de andiroba (Carapa guianensis)
• Beneficiário: Gislaine Cristina Roma
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores