Quantification of Methomyl Levels in Cabbage, Tomato, and Soya Milk Using a Renewable Amperometric Biosensor

Autor(es)

Caetano, Josiane ^{[1, 2]} ; Dragunski, Douglas C. ^[3] ; Pedrosa, Valber A. ^[4] ; Machado, Sergio A. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Estadual Oeste Parana, BR-85903000 Toledo, Parana - Brazil

^[3] Univ Paranaense, UNIPAR, Umuarama, PR - Brazil

^[4] UNESP, Inst Biosci, Dept Chem & Biochem, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Electrochemical Science; v. 8, n. 6, p. 7795-7805, JUN 2013.
• Citações Web of Science: 6

Resumo

An enzymatic biosensor based on inhibition of acetylcholinesterase activity was developed to analyze methomyl contamination in different matrices. The detection limits under optimal working conditions were found to be 30.4 mu g L-1 methomyl using the biosensor and 0.15 mu g L-1 using HPLC. The biosensor was then applied to analysis of methomyl in fruit and vegetable samples at concentrations in the ppb range in the absence of sample pretreatment. Recovery levels using the biosensor were effective, ranging from 78.0 to 96.5%, while the HPLC method yielded 57.0 to 99.5% recovery. Results for total carbamate concentrations obtained using the acetylcholinesterase biosensor were compared to those obtained using HPLC. (AU)

• Processo FAPESP: 06/51625-7 - Desenvolvimento, caracterizacao e aplicacoes iniciais de superficies nanoestruturadas para a confeccao de nanobiossensores.
• Beneficiário: Sergio Antonio Spinola Machado
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 03/07653-8 - Desenvolvimento e testes de eletrodos modificados com enzimas colinesterase como sensor para a deteccao de pesticidas em matrizes de alimentos.
• Beneficiário: Josiane Caetano Dragunski
• Modalidade de apoio: Bolsas no Brasil - Doutorado