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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Quantification of Methomyl Levels in Cabbage, Tomato, and Soya Milk Using a Renewable Amperometric Biosensor

Author(s):
Caetano, Josiane [1, 2] ; Dragunski, Douglas C. [3] ; Pedrosa, Valber A. [4] ; Machado, Sergio A. S. [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil
[2] Univ Estadual Oeste Parana, BR-85903000 Toledo, Parana - Brazil
[3] Univ Paranaense, UNIPAR, Umuarama, PR - Brazil
[4] UNESP, Inst Biosci, Dept Chem & Biochem, Botucatu, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: International Journal of Electrochemical Science; v. 8, n. 6, p. 7795-7805, JUN 2013.
Web of Science Citations: 6
Abstract

An enzymatic biosensor based on inhibition of acetylcholinesterase activity was developed to analyze methomyl contamination in different matrices. The detection limits under optimal working conditions were found to be 30.4 mu g L-1 methomyl using the biosensor and 0.15 mu g L-1 using HPLC. The biosensor was then applied to analysis of methomyl in fruit and vegetable samples at concentrations in the ppb range in the absence of sample pretreatment. Recovery levels using the biosensor were effective, ranging from 78.0 to 96.5%, while the HPLC method yielded 57.0 to 99.5% recovery. Results for total carbamate concentrations obtained using the acetylcholinesterase biosensor were compared to those obtained using HPLC. (AU)