The transcription factor chicken Scratch2 is expressed in a subset of early postmitotic neural progenitors

Autor(es)

Vieceli, Felipe Monteleone ^[1] ; Simoes-Costa, Marcos ^[2] ; Turri, Jose Antonio ^[1] ; Kanno, Tatiane ^[1] ; Bronner, Marianne ^[2] ; Yan, Chao Yun Irene ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Cell & Dev Biol, Sao Paulo - Brazil

^[2] CALTECH, Div Biol, Pasadena, CA 91125 - USA

• Tipo de documento: Artigo Científico
• Fonte: GENE EXPRESSION PATTERNS; v. 13, n. 5-6, p. 189-196, JUN-JUL 2013.
• Citações Web of Science: 3

Resumo

Scratch proteins are members of the Snail superfamily which have been shown to regulate invertebrate neural development. However, in vertebrates, little is known about the function of Scratch or its relationship to other neural transcription factors. We report the cloning of chicken Scratch2 (cScrt2) and describe its expression pattern in the chick embryo from HH15 through HH29. cScrt2 was detected in cranial ganglia, the nasal placode and neural tube. At all stages examined, cScrt2 expression is only detected within a subregion of the intermediate zone of the neural tube. cScrt2 is also expressed in the developing dorsal root ganglia from HH22-23 onwards and becomes limited to its dorsal medial domain at HH29. phospho-Histone H3 and BrdU-labeling revealed that the cScrt2 expression domain is located immediately external to the proliferative region. In contrast, cScrt2 domain overlapped almost completely with that of the postmitotic neural transcription factor NeuroM/Ath3/NEUROD4. Together, these data define cScrt2-positive cells as a subset of immediately postmitotic neural progenitors. Previous data has shown that Scrt2 is a repressor of E-box-driven transcription whereas NeuroM is an E-box-transactivator. In light of these data, the co-localization detected here suggests that Scrt2 and NeuroM may have opposing roles during definition of neural subtypes. (C) 2013 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 09/50544-1 - Expressão e função do fator de transcrição cScratch na neurogênese do gânglio da raiz dorsal e no tubo neural
• Beneficiário: Chao Yun Irene Yan
• Modalidade de apoio: Auxílio à Pesquisa - Regular