| Texto completo | |
| Autor(es): |
Afonso, Andre S.
[1, 2]
;
Zanetti, Bianca F.
[3]
;
Santiago, Adelita C.
[3]
;
Henrique-Silva, Flavio
[3]
;
Mattoso, Luiz H. C.
[2]
;
Faria, Ronaldo C.
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Fed Sao Carlos, Dept Quim, BR-13565905 Sao Carlos, SP - Brazil
[2] Embrapa Instrumentacao Agr, BR-13560970 Sao Carlos, SP - Brazil
[3] Univ Fed Sao Carlos, Dept Genet & Evolucao, BR-13565905 Sao Carlos, SP - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Talanta; v. 104, p. 193-197, JAN 30 2013. |
| Citações Web of Science: | 10 |
| Resumo | |
Citrus canker is one of the most important agricultural citrus diseases worldwide. It is caused by Xanthomonas citri subsp. citri (Xcc) bacterium that infects leaves and the fruits produce a cysteine peptidase (CPXaC), which makes it a potential target for the development of effective and rapid detection methods for citrus canker. We report here the studies on the development of piezoelectric immunoassay for CPXaC using a polyclonal antibody against CPXaC (anti-CPXaC). Three different strategies for covalent immobilization of anti-CPXaC on gold surfaces were evaluated by monitoring the frequency (Delta f) and energy dissipation (Delta D) variation in real time when 64.5 x 10(-8) mol L-1 CPXaC was added. Anti-CPXaC immobilized with 11-mercaptoundecanoic acid (MUA) showed the best relation between the frequency and dissipation factor variation, and strong values for the kinetic and equilibrium binding constant were obtained. The immunosensor showed a detection limit of 13.0 nmol L-1 with excellent specificity, showing no response for different proteins that include another cysteine peptidase that is used as a target to detect Xylella fastidiosa bacterium, responsible for another important citrus disease. These results provide good perspectives for the use of CPXaC as a new biomarker for citrus canker. (C) 2012 Elsevier B.V. All rights reserved. (AU) | |
| Processo FAPESP: | 11/02259-6 - Desenvolvimento de biossensores para determinação de biomarcadores proteicos visando à aplicação no diagnóstico precoce e monitoramento de câncer |
| Beneficiário: | Ronaldo Censi Faria |
| Modalidade de apoio: | Bolsas no Exterior - Pesquisa |