The mitochondrial genome of the blowfly Chrysomya chloropyga (Diptera: Calliphoridae)

Autor(es)

Junqueira, Ana Carolina M. ; Lessinger, Ana Cláudia ; Torres, Tatiana Teixeira ; Silva, Felipe Rodrigues da ; Vettore, André Luiz ; Arruda, Paulo ; Azeredo Espin, Ana Maria L. ^[7]

• Tipo de documento: Artigo Científico
• Fonte: Gene; v. 339, n. 1/2, p. 7-15, Sept. 2004.
• Área do conhecimento: Ciências Biológicas - Genética
• Assunto(s): Moscas

Resumo

In view of the medical, sanitary and forensic importance of Chrysomya species, a knowledge of their nucleotide sequences would be useful for the molecular characterization of this genus, and would help in designing primers and in improving the molecular identification of Calliphoridae species. In this work, the mitochondrial genome of the blowfly Chrysomya chloropyga (Diptera: Calliphoridae) was completely sequenced. The entire mitochondrial DNA (mtDNA) molecule was 15,837 bp long and was sequenced using the shotgun approach. The overall nucleotide composition was heavily biased towards As and Ts, which accounted for 76.7% of the whole genome. The cox1 gene had a serine as the start codon, while incomplete termination codons mediated by tRNA signals were found for cox2, nd4 and nd5. The C. chloropyga genes were in the same order and orientation as the mitochondrial genome of other dipteran species, except for the occurrence of a 123 bp region that included a complete duplication of tRNAIle and a partial duplication of tRNAGln genes. C. chloropyga is the first species of Diptera with 23 tRNA genes instead of the usual 22 already described. A phylogenetic analysis showed a split of Brachycera into Calyptratae and Acalyptratae subdivisions. The complete sequence of C. chloropyga mtDNA described here will be a useful source of sequence information for general molecular and evolutionary studies in Diptera. (AU)

• Processo FAPESP: 00/13814-6 - Análise comparativa das variações do DNA mitocondrial e morfológicas de espécies do gênero Chrysomya (Diptera: Calliphoridae) no Brasil
• Beneficiário: Ana Maria Lima de Azeredo-Espin
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 03/05444-2 - Evolução molecular do genoma mitocondrial da família Calliphoridae (Diptera: Brachycera)
• Beneficiário: Ana Carolina Martins Junqueira
• Modalidade de apoio: Bolsas no Brasil - Doutorado