Immune response to native NadA from Neisseria meningitidis and its expression in clinical isolates in Brazil

Autor(es)

Fukasawa, Lucila O. ; Gorla, Maria Cecília O. ; Lemos, Ana Paula S. ; Schenkman, Rocilda P. F. ; Brandileone, Maria Cristina C. ; Fox, Jay W. ; Raw, Isaias ; Frasch, Carl E. ; Tanizaki, Martha M. ^[9]

• Tipo de documento: Artigo Científico
• Fonte: Journal of Medical Microbiology; v. 52, n. 2, p. 121-125, Feb. 2003.
• Área do conhecimento: Ciências Biológicas - Microbiologia
• Assunto(s): Antígenos de bactérias Proteínas Bactérias Neisseria meningitidis Vacinação

Resumo

A mAb against the NadA protein from Neisseria meningitidis strain 3006 (serosubtype B : 2b : P1.2 : P5.2,8) demonstrated strong bactericidal activity against Brazilian epidemic serogroup B strain N44/89 (B : 4,7 : P1.19,15 : P5.5,7) and a serogroup C strain, IMC 2135 (C : 2a : P1.5,2), but not against another serogroup C strain, N1002/90 (C : 2b : P1.3 : P5.8). The immunogenicity of native NadA in an outer-membrane vesicle (OMV) preparation was also tested. Serum from mice immunized with OMV from serogroup B strain N44/89, which contains the NadA protein, showed bactericidal activity against serogroup B and C strains possessing NadA. In dotblot analysis of 100 serogroup B and 100 serogroup C isolates from Brazilian patients, the mAb to NadA recognized about 60% of the samples from both serogroups. The molecular mass of the NadA protein from strain N44/89 determined by mass spectrometry was 37 971 Da and the peptide sequences were identical to those of NadA from N. meningitidis strain MC58. (AU)

• Processo FAPESP: 00/08464-6 - Vacinas conjugadas anti-meningite: da bancada para escala piloto
• Beneficiário: Martha Massako Tanizaki
• Modalidade de apoio: Auxílio à Pesquisa - Regular