| Full text | |
| Author(s): |
Fukasawa, Lucila O.
;
Gorla, Maria Cecília O.
;
Lemos, Ana Paula S.
;
Schenkman, Rocilda P. F.
;
Brandileone, Maria Cristina C.
;
Fox, Jay W.
;
Raw, Isaias
;
Frasch, Carl E.
;
Tanizaki, Martha M.
[9]
Total Authors: 9
|
| Document type: | Journal article |
| Source: | Journal of Medical Microbiology; v. 52, n. 2, p. 121-125, Feb. 2003. |
| Field of knowledge: | Biological Sciences - Microbiology |
| Abstract | |
A mAb against the NadA protein from Neisseria meningitidis strain 3006 (serosubtype B : 2b : P1.2 : P5.2,8) demonstrated strong bactericidal activity against Brazilian epidemic serogroup B strain N44/89 (B : 4,7 : P1.19,15 : P5.5,7) and a serogroup C strain, IMC 2135 (C : 2a : P1.5,2), but not against another serogroup C strain, N1002/90 (C : 2b : P1.3 : P5.8). The immunogenicity of native NadA in an outer-membrane vesicle (OMV) preparation was also tested. Serum from mice immunized with OMV from serogroup B strain N44/89, which contains the NadA protein, showed bactericidal activity against serogroup B and C strains possessing NadA. In dotblot analysis of 100 serogroup B and 100 serogroup C isolates from Brazilian patients, the mAb to NadA recognized about 60% of the samples from both serogroups. The molecular mass of the NadA protein from strain N44/89 determined by mass spectrometry was 37 971 Da and the peptide sequences were identical to those of NadA from N. meningitidis strain MC58. (AU) | |