Molecular technics to evaluate serotypes/serogroup and antibiotic resistance genes in bacterial meningitis in the ABC region of São Paulo state

• Grant number: 17/03022-6
• Support Opportunities: Regular Research Grants
• Duration: September 01, 2017 - June 30, 2021
• Field of knowledge: Health Sciences - Collective Health - Public Health
• Principal Investigator: Ivana Barros de Campos
• Grantee: Ivana Barros de Campos
• Host Institution: Instituto Adolfo Lutz (IAL). Coordenadoria de Controle de Doenças (CCD). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: Andréia Moreira dos Santos Carmo ; Maria Cecília Cergole Novella
• Associated scholarship(s): 21/01194-0 - Molecular technics to evaluate serotypes/serogroups and antibiotic resistance genes in bacterial meningitis in the ABC region of São Paulo state, BP.TT; 18/22718-4 - Molecular techniques to evaluate serotypes/serogroups and antibiotic resistance genes in bacterial meningitis in the ABC region of São Paulo State, BP.TT

Abstract

Bacterial meningitis is a disease of great impact in Public Health, and is caused mainly by Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae. These bacteria have great variation in their polysaccharide capsule, which differentiates them in serogroups or serotypes. The available vaccines are composed of capsular polysaccharides conjugated to a carrier protein. They are effective in inducing memory immune response, however, they generate serotype/serogroup-dependent response and thus may promote replacement of serotypes/serogroups in population. Therefore, it is very important the constant surveillance of circulating strains to determine strategies to combat the agents that cause bacterial meningitis, such as modification in the vaccination schedules or the composition of vaccine offered to the population. In order to determine the serotypes/serogroups of the bacterial etiological agents, techniques such as the agglutination reaction are used. Despite providing the necessary information, they require specific antisera that represent a high cost, besides being very time-consuming and the results are subjectively interpreted. Therefore, the use of modern techniques, such as the polymerase chain reaction (PCR) technique, has been applied to identify the species causing meningitis as well as their serotypes/serogroups. PCR has high sensitivity and specificity, produces faster results because it does not require previous growth of the microorganism and provides more complete information than the standard techniques. Therefore, this project has as main objective to test the PCR technique to evaluate the serotypes/serogroups of the 3 main bacteria that cause meningitis in the 7 cities of the great São Paulo, known as the great ABC, which were attended by the Adolfo Lutz Institute of Santo André. This could promote important epidemiological information for the region. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE MATTOS, ELAINE CRISTINA; MATSUDA, ELAINE MONTEIRO; COLPAS, DANIELA RODRIGUES; CARVALHO, FLAVIA; CARMO, ANDREIA MOREIRA DOS SANTOS; DAROS, VILMA DOS SANTOS MENEZES GAIOTTO; DE CAMPOS, IVANA BARROS. Can urine be a potential biohazard in times of SARS-CoV-2 pandemic?. Journal of Medical Virology, v. 93, n. 3, NOV 2020. (17/03022-6)

MATSUDA, ELAINE MONTEIRO; OZORIO COELHO, LUANA PORTES; AHAGON, CINTIA MAYUMI; CAMPOS, IVANA BARROS; DE MACEDO BRIGIDO, LUIS FERNANDO. Immediate start of antiretroviral, why not?. Brazilian Journal of Infectious Diseases, v. 22, n. 3, p. 250-251, MAY-JUN 2018. (16/14813-1, 17/03022-6)

SOUZA, MARIANA BRENA; CERGOLE-NOVELLA, MARIA CECILIA; MOLINARI, DELMA APARECIDA; COLPAS, DANIELA RODRIGUES; DOS SANTOS CARMO, ANDREIA MOREIRA; MENEZES GAIOTTO DAROS, VILMA DOS SANTOS; DE CAMPOS, IVANA BARROS. Multiplex real-time PCR using SYBR Green: Unspecific intercalating dye to detect antimicrobial resistance genes of Streptococcus pneumoniae in cerebrospinal fluid. PLoS One, v. 17, n. 6, p. 14-pg., 2022-01-01. (18/22718-4, 17/03022-6)