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Molecular epidemiology of gram-negative bacteria and genetic of antibiotic resistance

Grant number: 14/14494-8
Support type:Research Projects - Thematic Grants
Duration: May 01, 2015 - October 31, 2019
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Ana Lúcia da Costa Darini
Grantee:Ana Lúcia da Costa Darini
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Assoc. researchers:Alessandro de Mello Varani ; Lidia Alice Gomes Monteiro Marin Torres ; Rafael Antonio Casarin Penha Filho
Associated scholarship(s):15/23484-9 - Molecular study of gram-negative beta-lactamase producing bacteria isolated from patients with suspected meningitis in the Ribeirão Preto region, BP.DD
15/11728-0 - Complete sequencing of plasmids carrying antibiotic resistance genes in bacteria of interest and genome analysis of Pseudomonas aeruginosa-producing SPM-1 and KPC-2 lines by large-scale sequencing, BP.PD

Abstract

There is growing concern about bacteria that cause hospital-acquired infections or in the community, especially the ones multiresistant to antibiotics (popularly named superbacteria) or those affecting patients with chronic infectious diseases. Therefore, this proposed Thematic Project covers highly specialized and innovative studies on genetics of antibiotic resistance in bacteria, investigation on virulence associated with resistance and epidemiology of bacterial infections in Brazil. The first four subprojects plus subprojects 7 and 8 aimed directly to explore the spread of plasmidial resistance and/or virulence genes which can contribute to the successful colonization/infection of the bacteria and/or exacerbation of bacterial infections: (1) Phenotypic and molecular characterization of antibiotic resistant enterobacteria isolated from broiler chickens in farms from Sao Paulo and Goiás State, (2) Molecular study of beta-lactamases-producing Gram-negative bacteria isolated from patients with suspected meningitis in the region of Ribeirão Preto, (3) Typing of plasmid carrying blaOXA genes in Acinetobacter spp. by A. baumannii-PCR Based Replicon Typing, (4) Entire sequencing of resistance plasmids carrying resistance genes in gram-negative bacteria, (7) Investigation of virulence, resistance, and populational structure of Enterobacter cloacae Complex and (8) Genome analyses of SPM-1- and KPC-2-producing Pseudomonas aeruginosa by next generation sequencing. Finally the fifth and sixth subprojects aimed at the molecular study of bacteria complicating the clinical status of patients with cystic fibrosis: (5) Phenotypic and molecular characterization of Pseudomonas aeruginosa isolated from patients with cystic fibrosis and clinical evaluation of the patients and (6) Epidemiology of bacterial infections in patients with cystic fibrosis involving Achromobacter spp. and Burkholderia cepacia complex. This thematic project encompasses multidisciplinary, multi-institutional and transnational team, with proven expertise in different areas of the subprojects interconnected by common mottos. (AU)

Scientific publications (14)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CASARIN PENHA FILHO, RAFAEL ANTONIO; FERREIRA, JOSEANE CRISTINA; IBA KANASHIRO, ANA MARIA; JUNIOR, ANGELO BERCHIERI; DA COSTA DARINI, ANA LUCIA. Emergent multidrug-resistant nontyphoidal Salmonella serovars isolated from poultry in Brazil coharboring bia(CTX-M-2) and qnrB or bla(CMY-2) in large plasmids. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 95, n. 1, p. 93-98, SEP 2019. Web of Science Citations: 0.
FERREIRA, JOSEANE CRISTINA; CASARIN PENHA FILHO, RAFAEL ANTONIO; ANDRADE, LEONARDO NEVES; COSTA DARINI, ANA LUCIA. Evaluation of heavy metal tolerance genes in plasmids harbored in multidrug-resistant Salmonella enterica and Escherichia coli isolated from poultry in Brazil. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 94, n. 3, p. 314-315, JUL 2019. Web of Science Citations: 2.
GALETTI, RENATA; ANDRADE, LEONARDO NEVES; VARANI, ALESSANDRO M.; COSTA DARINI, ANA LUCIA. A Phage-Like Plasmid Carrying bla(KPC-2) Gene in Carbapenem-Resistant Pseudomonas aeruginosa. FRONTIERS IN MICROBIOLOGY, v. 10, MAR 20 2019. Web of Science Citations: 0.
GALETTI, RENATA; CASARIN PENHA FILHO, RAFAEL ANTONIO; FERREIRA, JOSEANE CRISTINA; VARANI, ALESSANDRO M.; COSTA DARINI, ANA LIICIA. Antibiotic resistance and heavy metal tolerance plasmids: the antimicrobial bulletproof properties of Escherichia fergusonii isolated from poultry. INFECTION AND DRUG RESISTANCE, v. 12, p. 1029-1033, 2019. Web of Science Citations: 1.
CACADOR, NATALIA CANDIDO; DA COSTA CAPIZZANI, CAROLINA PAULINO; MONTEIRO MARIN TORRES, LFDIA ALICE GOMES; GALETTI, RENATA; CIOFU, OANA; DA COSTA DARINI, ANA LTICIA; FLEFIBY, NIELS. Adaptation of Pseudomonas aeruginosa to the chronic phenotype by mutations in the algTmucABD operon in isolates from Brazilian cystic fibrosis patients. PLoS One, v. 13, n. 11 NOV 29 2018. Web of Science Citations: 3.
FERREIRA, JOSEANE CRISTINA; CASARIN PENHA FILHO, RAFAEL ANTONIO; YORIKA KUAYE, ANA PAULA; ANDRADE, LEONARDO NEVES; CHANG, YUNG-FU; COSTA DARINI, ANA LUCIA. Virulence potential of commensal multidrug resistant Escherichia coli isolated from poultry in Brazil. INFECTION GENETICS AND EVOLUTION, v. 65, p. 251-256, NOV 2018. Web of Science Citations: 2.
FERREIRA, JOSEANE CRISTINA; CASARIN PENHA FILHO, RAFAEL ANTONIO; YORIKA KUAYE, ANA PAULA; ANDRADE, LEONARDO NEVES; BERCHIERI JUNIOR, ANGELO; DA COSTA DARINI, ANA LUCIA. Identification and characterization of plasmid-mediated quinolone resistance determinants in Enterobacteriaceae isolated from healthy poultry in Brazil. INFECTION GENETICS AND EVOLUTION, v. 60, p. 66-70, JUN 2018. Web of Science Citations: 8.
DA COSTA CAPIZZANI, CAROLINA PAULINO; CACADOR, NATALIA CANDIDO; MARQUES, ELIZABETH ANDRADE; LEVY, CARLOS EMILIO; TONANI, LUDMILLA; MONTEIRO MARIN TORRES, LIDIA ALICE GOMES; DA COSTA DARINI, ANA LUCIA. A practical molecular identification of nonfermenting Gram-negative bacteria from cystic fibrosis. Brazilian Journal of Microbiology, v. 49, n. 2, p. 422-428, APR-JUN 2018. Web of Science Citations: 1.
ANDRADE, LEONARDO N.; SIQUEIRA, THIAGO E. S.; MARTINEZ, ROBERTO; DARINI, ANA LUCIA C. Multidrug-Resistant CTX-M-(15,9,2)- and KPC-2-Producing Enterobacter hormaechei and Enterobacter asburiae Isolates Possessed a Set of Acquired Heavy Metal Tolerance Genes Including a Chromosomal sil Operon (for Acquired Silver Resistance). FRONTIERS IN MICROBIOLOGY, v. 9, MAR 23 2018. Web of Science Citations: 11.
DA COSTA CAPIZZANI, C. P.; CACADOR, N. C.; TORRES, L. A. G. M. M.; TONANI, L.; VANDAMME, P.; DA COSTA DARINI, A. L. Clinical and microbiological profile of chronic Burkholderia cepacia complex infections in a cystic fibrosis reference hospital in Brazil. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, v. 36, n. 11, p. 2263-2271, NOV 2017. Web of Science Citations: 2.
FERREIRA, JOSEANE CRISTINA; CASARIN PENHA FILHO, RAFAEL ANTONIO; ANDRADE, LEONARDO NEVES; BERCHIERI JUNIOR, ANGELO; COSTA DARINI, ANA LUCIA. Diversity of plasmids harboring bla(CMY-2) in multidrug-resistant Escherichia coli isolated from poultry in Brazil. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 88, n. 4, p. 361-364, AUG 2017. Web of Science Citations: 5.
FERREIRA, JOSEANE CRISTINA; CASARIN PENHA FILHO, RAFAEL ANTONIO; ANDRADE, LEONARDO NEVES; BERCHIERI JUNIOR, ANGELO; COSTA DARINI, ANA LUCIA. Evaluation and characterization of plasmids carrying CTX-M genes in a non-clonal population of multidrug-resistant Enterobacteriaceae isolated from poultry in Brazil. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 85, n. 4, p. 444-448, AUG 2016. Web of Science Citations: 11.
GALETTI, RENATA; ANDRADE, LEONARDO NEVES; CHANDLER, MICHAEL; VARANI, ALESSANDRO DE MELLO; COSTA DARINI, ANA LUCIA. New Small Plasmid Harboring bla(KPC-2) in Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy, v. 60, n. 5, p. 3211-3214, MAY 2016. Web of Science Citations: 4.
FERREIRA, JOSEANE CRISTINA; CASARIN PENHA FILHO, RAFAEL ANTONIO; ANDRADE, LEONARDO NEVES; BERCHIERI JUNIOR, ANGELO; COSTA DARINI, ANA LUCIA. IncIl/ST113 and IncIl/ST114 conjugative plasmids carrying bla(CTX-M-8) in Escherichia coli isolated from poultry in Brazil. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 80, n. 4, p. 304-306, DEC 2014. Web of Science Citations: 12.

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