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Molecular study of gram-negative beta-lactamase producing bacteria isolated from patients with suspected meningitis in the Ribeirão Preto region

Grant number: 15/23484-9
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): December 01, 2015
Effective date (End): April 03, 2019
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Ana Lúcia da Costa Darini
Grantee:Anelise Stella Ballaben
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated research grant:14/14494-8 - Molecular epidemiology of gram-negative bacteria and genetic of antibiotic resistance, AP.TEM
Associated scholarship(s):17/11707-9 - Molecular investigation of high-level aminoglycoside resistance among gram-negative bacilli isolated from cerebrospinal fluid and blood of hospitalized patients in Brazil, BE.EP.DD


Although there are few reports regarding Enterobacteriaceae and Non-Fermenting Gram-Negative (BGN-NF) bacilli causing meningitis, these bacteria can both cause community and/or hospitalar meningitis. The treatment of the patients is compromised since it is increasingly common strains which have important resistance mechanisms, for example, production of Extended Spectrum Beta-Lactamase Enzymes (ESBL), AmpC and carbapenemases. It is known that several plasmidial resistance genes are widespread among the Enterobacteriaceae family and also among non-fermenting gram-negative bacilli. It is of great importance the detection and identification of these plasmidial genes for therapeutics, in order to increase the life expectancy and/or the improvement of the patient's life quality affected by the pathogen. The aim of this work is the phenotypic and molecular study of the production of ESBL, AmpC and carbapenemase in Enterobacteriaceae and BGN-NF isolated from patients with suspected meningitis in the region of Ribeirão Preto, which were previously investigated at the Instituto Adolfo Lutz of Ribeirão Preto, after 2006. It will be necessary to establish the sensitivity to beta-lactam antibiotics, phenotypic detection of ESBL production by the isolates, identification of ESBL, carbapenemase and AmpC encoding genes involved in the phenotype of resistance to beta-lactam antibiotics, evaluate probable concomitant presence of ESBL and other beta-lactamases and investigate the incompatibility group of plasmids present in these bacteria as well as the size and number of these plasmids. (AU)