| Texto completo | |
| Autor(es): Mostrar menos - |
Papini-Terzi, Flavia S.
[1]
;
Rocha, Flavia R.
[1]
;
Vencio, Ricardo Z. N.
[2]
;
Felix, Juliana M.
[3, 4]
;
Branco, Diana S.
[3]
;
Waclawovsky, Alessandro J.
[1]
;
Del Bem, Luiz E. V.
[3]
;
Lembke, Carolina G.
[1]
;
Costa, Maximiller D. L.
[1]
;
Nishiyama, Jr., Milton Y.
[1]
;
Vicentini, Renato
[4, 5]
;
Vincentz, Michel G. A.
[3, 5]
;
Ulian, Eugenio C.
[6, 4]
;
Menossi, Marcelo
[5]
;
Souza, Glaucia M.
[1]
Número total de Autores: 15
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-01498 Sao Paulo - Brazil
[2] Univ Sao Paulo, BIOINFO Nucleo Pesquisas Bioinformat, BR-01498 Sao Paulo - Brazil
[3] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Campinas, SP - Brazil
[4] Ctr Tecnol Canavieira, Piracicaba, SP - Brazil
[5] Univ Estadual Campinas, Inst Biol, Dept Genet & Evolucao, Campinas, SP - Brazil
[6] Monsanto Brasil Ltda, Sao Paulo - Brazil
Número total de Afiliações: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BMC Genomics; v. 10, p. 0-0, Mar. 2009. |
| Área do conhecimento: | Ciências Biológicas - Genética |
| Notas: | Revista de acesso aberto, artigo número 120. Os beneficiários do projeto são Flavia Stal Papini Terzi e Marcelo Menossi e fazem parte da autoria do trabalho. O artigo possui mais de dez autores |
| Citações Web of Science: | 65 |
| Assunto(s): | Expressão gênica DNA complementar Cana-de-açúcar Lignina Proteínas Aquaporinas |
| Resumo | |
Sucrose content is a highly desirable trait in sugarcane as the worldwide demand for cost-effective biofuels surges. Sugarcane cultivars differ in their capacity to accumulate sucrose and breeding programs routinely perform crosses to identify genotypes able to produce more sucrose. Sucrose content in the mature internodes reach around 20% of the culms dry weight. Genotypes in the populations reflect their genetic program and may display contrasting growth, development, and physiology, all of which affect carbohydrate metabolism. Few studies have profiled gene expression related to sugarcane's sugar content. The identification of signal transduction components and transcription factors that might regulate sugar accumulation is highly desirable if we are to improve this characteristic of sugarcane plants. We have evaluated thirty genotypes that have different Brix (sugar) levels and identified genes differentially expressed in internodes using cDNA microarrays. These genes were compared to existing gene expression data for sugarcane plants subjected to diverse stress and hormone treatments. The comparisons revealed a strong overlap between the drought and sucrose-content datasets and a limited overlap with ABA signaling. Genes associated with sucrose content were extensively validated by qRT-PCR, which highlighted several protein kinases and transcription factors that are likely to be regulators of sucrose accumulation. The data also indicate that aquaporins, as well as lignin biosynthesis and cell wall metabolism genes, are strongly related to sucrose accumulation. Moreover, sucrose-associated genes were shown to be directly responsive to short term sucrose stimuli, confirming their role in sugar-related pathways... (AU) | |
| Processo FAPESP: | 05/58104-0 - Localizacao subcelular de proteinas de cana-de-acucar (saccharum spp.): caracterizacao in silico e avaliacao funcional em larga escala. |
| Beneficiário: | Marcelo Menossi Teixeira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 02/01722-5 - Análise do transcriptoma da cana-de-açúcar utilizando-se a tecnologia de Microarrays de DNA |
| Beneficiário: | Flavia Stal Papini Terzi |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |