Interaction of organophosphorus pesticides with DNA nucleotides on a boron-doped diamond electrode

Autor(es)

Gustavo S. Garbellini ^[1] ; Carolina V. Uliana ^[1] ; Hideko Yamanaka ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Chem, Dept Analyt Chem, BR-14800900 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 24, n. 12, p. 1942-1949, 2013-12-00.

Resumo

Diamond electrode was used to evaluate the interaction of the nucleotides guanosine monophosphate (GMP) and adenosine monophosphate (AMP) with the pesticides chlorpyrifos, methamidophos and monocrotophos. Changes were observed in the currents and peak potentials of the nucleotide voltammograms in the presence of the pesticides, with dependence on the pesticide concentration (from 5.0 × 10-7 to 5.0 × 10-5 mol L-1) and the interaction time (from 1 min to 4 h). This is probably due to binding of the pesticides to the nitrogenous bases present in the nucleotides, which could lead to problems in the DNA replication and biological functions of nucleotides. The pesticides showed stronger interaction with AMP than with GMP. Studies of the interaction of 50 µg mL-1 DNA with the pesticides (from 30 min to 4 h and from 1.0 × 10-6 to 6.0 × 10-5 mol L-1) did not reveal any peaks relating to double helix opening or DNA unwinding. (AU)

• Processo FAPESP: 08/08990-1 - Estudos da lesão ao DNA por corantes têxteis e da capacidade protetora de flavonóides empregando biossensor eletroquímico
• Beneficiário: Carolina Venturini Uliana
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/08161-8 - Avaliação de interações entre pesticidas organofosforados e DNA empregando biossensor eletroquímico
• Beneficiário: Gustavo Stoppa Garbellini
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado