Tissue microarray is a reliable method for immunohistochemical analysis of pleomorphic adenoma

Autor(es)

Fonseca, Felipe Paiva ^[1] ; Benevenuto de Andrade, Bruno Augusto ^[1] ; Carrinho Ayrosa Rangel, Ana Lucia ; Della Coletta, Ricardo ^[1] ; Lopes, Marcio Ajudarte ^[1] ; de Almeida, Oslei Paes ^[1] ; Vargas, Pablo Agustin ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY; v. 117, n. 1, p. 81-88, JAN 2014.
• Citações Web of Science: 20

Resumo

Objective. To determine the most adequate number and size of tissue microarray (TMA) cores for pleomorphic adenoma immunohistochemical studies. Study design. Eighty-two pleomorphic adenoma cases were distributed in 3 TMA blocks assembled in triplicate containing 1.0-, 2.0-, and 3.0-mm cores. Immunohistochemical analysis against cytokeratin 7, Ki67, p63, and CD34 were performed and subsequently evaluated with PixelCount, nuclear, and microvessel software applications. Results. The 1.0-mm TMA presented lower results than 2.0-and 3.0-mm TMAs versus conventional whole section slides. Possibly because of an increased amount of stromal tissue, 3.0-mm cores presented a higher microvessel density. Comparing the results obtained with one, two, and three 2.0-mm cores, there was no difference between triplicate or duplicate TMAs and a single-core TMA. Conclusions. Considering the possible loss of cylinders during immunohistochemical reactions, 2.0-mm TMAs in duplicate are a more reliable approach for pleomorphic adenoma immunohistochemical study. (AU)

• Processo FAPESP: 12/07519-9 - Semaphorinas e neuropilinas em tumores de glândulas salivares
• Beneficiário: Felipe Paiva Fonseca
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/53839-2 - Criação do Laboratório de Patologia Digital através do uso do escaneador de lâminas histológicas (Aperio® Scanscope CS)
• Beneficiário: Oslei Paes de Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 12/10781-7 - Análise da expressão de citoqueratinas, do padrão angiogênico, proliferativo e de conteúdo de DNA celular de tumores de glândulas salivares
• Beneficiário: Pablo Agustin Vargas
• Modalidade de apoio: Auxílio à Pesquisa - Regular