| Full text | |
| Author(s): |
Fonseca, Felipe Paiva
[1]
;
Benevenuto de Andrade, Bruno Augusto
[1]
;
Carrinho Ayrosa Rangel, Ana Lucia
;
Della Coletta, Ricardo
[1]
;
Lopes, Marcio Ajudarte
[1]
;
de Almeida, Oslei Paes
[1]
;
Vargas, Pablo Agustin
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Estadual Campinas, Piracicaba Dent Sch, Sao Paulo - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY; v. 117, n. 1, p. 81-88, JAN 2014. |
| Web of Science Citations: | 20 |
| Abstract | |
Objective. To determine the most adequate number and size of tissue microarray (TMA) cores for pleomorphic adenoma immunohistochemical studies. Study design. Eighty-two pleomorphic adenoma cases were distributed in 3 TMA blocks assembled in triplicate containing 1.0-, 2.0-, and 3.0-mm cores. Immunohistochemical analysis against cytokeratin 7, Ki67, p63, and CD34 were performed and subsequently evaluated with PixelCount, nuclear, and microvessel software applications. Results. The 1.0-mm TMA presented lower results than 2.0-and 3.0-mm TMAs versus conventional whole section slides. Possibly because of an increased amount of stromal tissue, 3.0-mm cores presented a higher microvessel density. Comparing the results obtained with one, two, and three 2.0-mm cores, there was no difference between triplicate or duplicate TMAs and a single-core TMA. Conclusions. Considering the possible loss of cylinders during immunohistochemical reactions, 2.0-mm TMAs in duplicate are a more reliable approach for pleomorphic adenoma immunohistochemical study. (AU) | |
| FAPESP's process: | 12/07519-9 - SEMAPHORINS AND NEUROPILINS IN SALIVARY GLAND TUMORS |
| Grantee: | Felipe Paiva Fonseca |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 09/53839-2 - Creation of a Digital Pathology Laboratory using a histological slidescanner |
| Grantee: | Oslei Paes de Almeida |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 12/10781-7 - Analysis of the expression of cytokeratins, angiogenic and proliferative patterns, and DNA content of salivary gland tumors |
| Grantee: | Pablo Agustin Vargas |
| Support Opportunities: | Regular Research Grants |