Analysis of the expression of cytokeratins, angiogenic and proliferative patterns, and DNA content of salivary gland tumors

• Grant number: 12/10781-7
• Support Opportunities: Regular Research Grants
• Duration: November 01, 2012 - January 31, 2015
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Pablo Agustin Vargas
• Grantee: Pablo Agustin Vargas
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Associated researchers: Ana Lúcia Carrinho Ayroza Rangel ; Fernanda Viviane Mariano Brum Corrêa ; Luiz Paulo Kowalski ; Oslei Paes de Almeida

Abstract

Salivary gland tumors is a highly heterogeneous group that accounts for 3 to 6% of all head and neck neoplasias, with an incidence ranging from 0.4 to 13.5 cases/100,000 individuals. Due to their clinicopathologic variability, the diagnosis of these tumors becomes a real challenge even for experient pathologists, which contributes for the discrete knowledgment concerning biologic factors involved in the clinical behavior of these lesions. Therefore, the characterization of structural proteins such as cytokeratins and proliferative biomarkers, as well as the identification of new factors and receptors involved in the angiogenic process and the definition of the cellular DNA content could significantly assist the diagnosis of these neoplasias and favor an improved prognostic determination of the affected patients. In the present study, using tissue microarray and immunohistochemistry techniques, and with the help of softwares and algorithms for digital analyses, the authors aim to characterize the expression pattern of cytokeratins 5/6, 7, 10, 18 and 34²12 in 367 salivary gland tumors, also evaluating the proliferative pattern of these tumors with the molecular markers Ki67, Mcm-2 and HOXB7, as well as analyzing the tumoral angiogenic features by evaluating the expression of CD34, CD105, neuropilins-1 and -2, class 3 semaphorins A, B and F and vascular endothelium growth factor (VEGF) 165, and the cellular DNA content by image cytometry, identifying the importance of these biological variables for diagnosis and prognostic determination of patients affected by this group of lesion. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FONSECA, FELIPE P.; BINGLE, LYNNE; SANTOS-SILVA, ALAN R.; LOPES, MARCIO A.; DE ALMEIDA, OSLEI P.; DE ANDRADE, BRUNO A. B.; MARIANO, FERNANDA V.; KOWALSKI, LUIZ P.; RANGEL, ANA L. C. A.; MARTINS, MANOELA D.; et al. Semaphorins and neuropilins expression in salivary gland tumors. JOURNAL OF ORAL PATHOLOGY & MEDICINE, v. 45, n. 2, p. 119-126, FEB 2016. (12/07519-9, 09/53839-2, 12/10781-7)

VASCONCELOS, ARTUR CUNHA; WAGNER, VIVIAN PETERSEN; MEURER, LUISE; VARGAS, PABLO AGUSTIN; DE SOUZA, LELIA BATISTA; FONSECA, FELIPE PAIVA; SQUARIZE, CRISTIANE HELENA; CASTILHO, ROGERIO MORAES; MARTINS, MANOELA DOMINGUES. Immunoprofile of c-MET/PI3K signaling in human salivary gland tumors. ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY, v. 120, n. 2, p. 238-247, AUG 2015. (12/10781-7)

FONSECA, FELIPE PAIVA; MONTANHANA BASSO, MARCOS PAULO; MARIANO, FERNANDA VIVIANE; KOWALSKI, LUIZ PAULO; LOPES, MARCIO AJUDARTE; MARTINS, MANOELA DOMINGUES; CARRINHO AYROZA RANGEL, ANA LUCIA; SANTOS-SILVA, ALAN ROGER; VARGAS, PABLO AGUSTIN. Vascular endothelial growth factor immunoexpression is increased in malignant salivary gland tumors. ANNALS OF DIAGNOSTIC PATHOLOGY, v. 19, n. 3, p. 169-174, JUN 2015. (12/07519-9, 12/17659-2, 09/53839-2, 12/10781-7)

FONSECA, FELIPE PAIVA; BENEVENUTO DE ANDRADE, BRUNO AUGUSTO; CARRINHO AYROSA RANGEL, ANA LUCIA; DELLA COLETTA, RICARDO; LOPES, MARCIO AJUDARTE; DE ALMEIDA, OSLEI PAES; VARGAS, PABLO AGUSTIN. Tissue microarray is a reliable method for immunohistochemical analysis of pleomorphic adenoma. ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY, v. 117, n. 1, p. 81-88, JAN 2014. (12/07519-9, 09/53839-2, 12/10781-7)

FONSECA, FELIPE PAIVA; BINGLE, LYNNE; SANTOS-SILVA, ALAN ROGER; LOPES, MARCIO AJUDARTE; COLETTA, RICARDO D.; BENEVENUTO DE ANDRADE, BRUNO AUGUSTO; MARIANO, FERNANDA VIVIANE; KOWALSKI, LUIZ PAULO; CARRINHO AYROZA RANGEL, ANA LUCIA; MARTINS, MANOELA DOMINGUES; et al. Immunoexpression of hoxb7 and hoxb9 in salivary gland tumours. JOURNAL OF ORAL PATHOLOGY & MEDICINE, v. 45, n. 9, p. 672-681, OCT 2016. (12/10781-7, 12/07519-9, 09/53839-2)

FONSECA, FELIPE PAIVA; SENA FILHO, MARCONDES; ALTEMANI, ALBINA; SPEIGHT, PAUL M.; VARGAS, PABLO AGUSTIN. Molecular signature of salivary gland tumors: potential use as diagnostic and prognostic marker. JOURNAL OF ORAL PATHOLOGY & MEDICINE, v. 45, n. 2, p. 101-110, FEB 2016. (12/07519-9, 13/15955-6, 12/10781-7)

PULIDO DIAZ, KATYA; GONDAK, ROGERIO; MARTINS, LEANDRO LIPORONI; DE ALMEIDA, OSLEI PAES; LEON, JORGE ESQUICHE; MARIANO, FERNANDA VIVIANE; ALTEMANI, ALBINA; VARGAS, PABLO AGUSTIN. Fatty acid synthase and Ki-67 immunoexpression can be useful for the identification of malignant component in carcinoma ex-pleomorphic adenoma. JOURNAL OF ORAL PATHOLOGY & MEDICINE, v. 48, n. 3, p. 232-238, MAR 2019. (15/07304-0, 12/10781-7)